Pentane-2,4-dione

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Adopted according to OECD SIDS (publicly available peer reviewed source). Original document not available.

Data source

Reference

Reference 1

Reference Type:

secondary source

Title:

Unnamed

Year:

2001

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

1986

Materials and methods

Principles of method if other than guideline:

Five male and five female mice were used per dose. The doses used were 579, 694, 833, 1000 and 1200 mg/kg bw and were applied by intraperitoneal injection. Animals were observed over a period of 3 days after administration.

GLP compliance:

yes

Limit test:

no

Test material

Test material information

Constituent 1

Details on test material:

Purity: >99.2%

Test animals

Species:

mouse

Strain:

not specified

Sex:

male/female

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

water

Doses:

579, 694, 833, 1000 and 1200 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: no data
- Necropsy of survivors performed: no data
- Other examinations performed: clinical signs, body weight

Statistics:

LD50: probit analysis

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

808 mg/kg bw

Based on:

test mat.

95% CL:

732 - 889

Mortality:

Analysis of variance testing indicated that there was no significant difference in the mortality response for the male and female animals. Thus the LD50 was calculated using probit analysis on the combined male and female mortality values to obtain a pooled LD50 value of 807.9 mg/kg. All animals administered the 1200 mg/kg dose died within 4 hours after injection. The dose of 1000 mg/kg bw produced 80 % and 60 % mortality incidence and 833 mg/kg produced 60 % and 100 % mortality incidence in male and female mice, respectively. The lowest dose to produce mortality was the 694 mg/kg bw dose level which produced 20 % mortality in male and female mice.

Clinical signs:

Acute clinical signs of toxicity including narcosis or lethargy were observed for all mice that survived the first few hours after dosing. Within 6 to 7 hours after injection, the animals dosed with 833 mg/kg or 1000 mg/kg bw had either recovered from narcosis or died. The animals that recovered appeared ataxic, exhibited splayed hind quarters, body tremors and lacked a righting reflex when dropped from a high of 6 inches.

Body weight:

No significant effects on body weights were observed.