L-Aspartic acid, N,N'-1,2-ethanediylbis-, sodium salt (1:3)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From June 29 to July 09, 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0, 100, 180, 320, 560 and 1000 mg/L
- Sampling method: samples from the control medium and the test solutions of 100, 320 and 1000 mg/L were taken at t = 0 h (newly prepared solutions) and t = 48 h (spent solutions).
- Sample storage conditions before analysis: samples of ca. 100 mL were taken in polyethylene bottles, preserved by addition of formol (1% final active concentration) and stored in a refrigerator

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water Flea
- Strain: no data
- Source: TNO
- Age at study initiation (mean and range, SD): one day
- Weight at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): no data
- Valve height at study initiation, for shell deposition study (mean and range, SD): no data
- Peripheral shell growth removed prior to test initiation: no data
- Method of breeding: no data
- Feeding during test
- Food type/Amount/Frequency: fed daily with ca. 4 x 10(9) algal cells (Chlorella) and ca. 0.13 g of yeast per 4 litre.

ACCLIMATION
- Acclimation period:no data
- Acclimation conditions (same as test or not): no data
- Type and amount of food: no data
- Feeding frequency: no data
- Health during acclimation (any mortality observed): no data

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

no data

Hardness:

224 mg/L of CaCO3

Test temperature:

20.1-20.9oC

pH:

Between 7.1 to 7.9

Dissolved oxygen:

The lowest value measured was 8.8 mg/L

Salinity:

no data

Nominal and measured concentrations:

Nominal concentrations: 0, 100, 180, 320, 560 and 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass flask
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 150 mL glass flask having 100 mL of test solution
- Aeration: no aeration
- Type of flow-through (e.g. peristaltic or proportional diluter): no data
- Renewal rate of test solution (frequency/flow rate):not renewed
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: no data


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: groundwater from a locality near Linschoten (The Netherlands)
- Total organic carbon: 1.9 mg/L
- Particulate matter: no data
- Metals: refer to table 2
- Pesticides: no data
- Chlorine: 2.8 mmol/L
- Alkalinity: no data
- Ca/mg ratio: 1.42/0.82
- Conductivity: no data
- Culture medium different from test medium: no data
- Intervals of water quality measurement: no data


OTHER TEST CONDITIONS
- Adjustment of pH: between 7.1 to 7.9
- Photoperiod: 16 h light - 8 h dark regime
- Light intensity: no data


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- mobility and condition (swimming behaviour, colour, respiratory function or any other visually observable morphological or behavioural criterion) assessed at 0, 24 and 48 h during the test.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: based on range finding study
- Range finding study
- Test concentrations: no data
- Results used to determine the conditions for the definitive study: no data

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Remarks:

at higher concentrations the daphnids swam slower and with irregular movements

Details on results:

Immobilization and other observations on the test animals-
There were no immobile animals in the control media or at any tested concentration. At 560 and 1000 mg/L, the daphnids swam slower and with irregular movements, and at 1000 mg/L they appeared paler than the controls (See Table 4 for details).

Table 3: Number of mobile animals in the control medium and in the test solutions at 0, 24 and 48 h.

Time (h)	Nominal concentration of the test substance (mg/L)
	0	100	180	320	560	1000
0	20	20	20	20	20	20
24	20	20	20	20	20	20
48	20	20	20	20	20	20

Table 4: Number of animals and their condition in the control media and in the test solutions during the test (a, b, c and d are replicates)

Time (h)

Nominal concentration of the test substance (mg/L)

0

100

180

0

5^a)

5^a)

5^a)

5^a)

5^a)

5^a)

5^a)

5^a)

5^a)

5^a)

5^a)

5^a)

24

5^a)

5^a)

5^a)

5^a)

5^b)

5^b)

5^b)

5^b)

5^b)

5^b)

5^b)

5^b)

48

5^a)

5^a)

5^a)

5^a)

5^b)

5^b)

5^b)

5^b)

5^b)

5^b)

5^b)

5^b)

320

560

1000

0

5^a)

5^a)

5^a)

5^a)

5^a)

5^a)

5^a)

5^a)

5^a)

5^a)

5^a)

5^a)

24

5^b)

5^b)

5^b)

5^b)

5^b)

5^b)

5^b)

5^b)

5^b)

5^b)

5^b)

5^b)

48

5^b)

5^b)

5^b)

5^b)

5^ef)

5^ef)

5^ef)

5^ef)

5^efg)

5^efg)

5^efg)

5^efg)

1) The following codes are used to denote condition:

a) Condition of all test animals, visually assessed, normal (= good).

b) Condition of all test animals visually assessed, equal to that of the control animals.

e) The quoted number of animals were mobile, according to the definition given in the OECD Guideline

no. 202, but their movements were slower.

f) The quoted number of animals were mobile, but they swam with irregular movements.

g) The quoted number of animals were mobile, but they were paler than the control animals

Validity criteria fulfilled:

yes

Conclusions:

In a GLP study conducted according to OECD Guideline 202, the 48-h NOEC of trisodium EDDS for mobility and swimming behaviour of the freshwater crustacean, Daphnia magna, was 1000 mg/L (the highest tested concentration) and 320 mg/L, respectively. After 48 h exposure, all daphnids were mobile at all the tested concentrations (therefore the 48-h EC50 would be >1000 mg/L).

Executive summary:

In a GLP study conducted according to OECD Guideline 202, the acute toxicity of trisodium EDDS to the fresh-water crustacean, Daphnia magna, was assessed. The test was carried out with 4 beakers each containing 5 daphnids for each test concentration, and the test solutions were not replaced. The exposure duration was 48 h. The animals were assessed for mobility and condition (swimming behaviour, colour, respiratory function or other visually observable morphological or behavioural criterion) at 0, 24 and 48 h. The nominal concentrations tested were 0, 100, 180, 320, 560 and 1000 mg/L.

All test solutions were completely clear (visually assessed) throughout the test. After 48 h exposure all daphnids were mobile at all the tested concentrations (therefore the 48-h EC50 would be >1000 mg/L). Regarding swimming behaviour, at 560 and 1000 mg/L the daphnids swam slower with irregular movements, and at 1000 mg/L they were paler in appearance than controls. Therefore, under the conditions of this study, the 48-h NOEC of trisodium EDDS for mobility and behaviour of D. magna were 1000 mg/L and 320 mg/L, respectively.

Description of key information

In a GLP study conducted according to OECD Guideline 202, the 48-h NOEC of trisodium EDDS for mobility and swimming behaviour of the freshwater crustacean, Daphnia magna, was 1000 mg/L (the highest tested concentration) and 320 mg/L, respectively.

After 48 h exposure, all daphnids were mobile at all the tested concentrations, therefore the 48-h EC50 would be >1000 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 1 000 mg/L

Additional information

In a GLP study conducted according to OECD Guideline 202, the acute toxicity of trisodium EDDS to the freshwater crustacean, Daphnia magna, was assessed. The test was carried out with 4 beakers each containing 5 daphnids for each test concentration, and the test solutions were not replaced. The exposure duration was 48 h. The animals were assessed for mobility and condition (swimming behaviour, colour, respiratory function or other visually observable morphological or behavioural criterion) at 0, 24 and 48 h. The nominal concentrations tested were 0, 100, 180, 320, 560 and 1000 mg/L. All test solutions were completely clear (visually assessed) throughout the test.

After 48 h exposure all daphnids were mobile at all the tested concentrations (therefore the 48-h EC50 would be >1000 mg/L). Regarding swimming behaviour, at 560 and 1000 mg/L the daphnids swam slower with irregular movements, and at 1000 mg/L they were paler in appearance than controls. Therefore, under the conditions of this study, the 48-h NOEC for trisodium EDDS for mobility and behaviour of Daphnia magna were 1000 mg/L and 320 mg/L, respectively (Hooftman and van Drongeien-Sevenhuijsen, 1993).

No data in marine invertebrates are currently available.