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Diss Factsheets
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EC number: 947-977-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- As the results of this range finding test are considered to be valid and because both test substances are very similar in their fate during the algae test, the conclusion as obtained from this test is considered sufficient to select the more toxic substance as the worst-case representative in the hazard assessment of both substances if the higher toxicity observed for substance 2 is confirmed in an daphnia range finding test.
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
See chapter 13 for more information on the Read across - Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- only range finding is performed to compare two substances
- GLP compliance:
- no
- Remarks:
- Range finding study to validate read across theory
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test medium Dutch Standard Water (DSW) was used for testing. DSW has a pH approximately 8 and contains: 200 mg of CaCl2•2H2O, 180 mg of MgSO4•7H2O, 100 mg of NaHCO3 and 20 mg of KHCO3.The water was made by an automatic dosing system.
The test substances were found to be readily soluble or dispersible in water and therefore stock solutions of 100 mg/L of test substances were prepared in test media as follows: To an accurately weighed amount of 0.0521 g of the test substances 400 mL of freshly prepared DSW medium was added. The stock solutions were then agitated with a magnetic stirrer until homogeneous solutions were achieved. Both test substances appeared to dissolve easily. pH was checked and was in both cases pH 8.25. The pH was therefore not adjusted. The total volume was then made up to 500 mL for both test chemicals. Clear homogeneous stocks were produced. The appropriate volume of the test substance stocks was then transferred to 100 mL volumetric flasks and further diluted with test media to generate the test concentrations indicated below. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The test animals were taken from a Daphnia magna clone 5 stock, (Origin: NOTOX B.V The
Netherlands) cultured in conformity with the relevant SOP. The animals used in the test were less than 24 hours old and were obtained from parent animals reproducing parthenogenically and having an age of 2-4 weeks (having previously produced at least one brood before use). The culture is checked yearly for sensitivity by a reference test with Potassium dichromate. The results of this test were within the range given in the relevant
guideline. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- DSW
- Test temperature:
- 18.8 - 20.1 °C
- pH:
- 8.1 - 8.2
- Dissolved oxygen:
- 8.8 - 8.9 mg/L
- Salinity:
- DSW
- Nominal and measured concentrations:
- Standard daphnia screening test concentrations of 0.01, 0.1, 1.0, 10 and 100 mg/L were prepared for both test substances in test media. (nominal)
- Reference substance (positive control):
- yes
- Remarks:
- The culture is checked yearly for sensitivity by a reference test with Potassium dichromate. The results of this test were within the range given in the relevant guideline.
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Substance 1
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Substance 2
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Substance 2
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- Within the guideline requirements
- Reported statistics and error estimates:
- -
- Validity criteria fulfilled:
- yes
- Remarks:
- for a range finding test
- Conclusions:
- The daphnia rangefinding test has been performed to evaluate which of both substances tested is the more toxic substance. The results obtained indicate that there is a slightly higher toxicity observed for test substance 2. As the results of this range finding test are considered to be valid and because both test substances are very similar in their fate during the daphnia test the conclusion as obtained from this test is considered sufficient to select the more toxic substance as the worst-case representative in the hazard assessment of both substances if the result obtained is confirmed in an algae range finding test.
- Executive summary:
The acute toxicity of two similar substances to daphnia magna was evaluated in a rangefinding test performed according to OECD 202.
For substance 1 ( A complex mixture which includes alkyl naphthalenes, alkyl biphenyls, and alkyl benzenes. low nonene substitution batch no S001235) a NOEC could be calculated of 100 mg/L test substance
For substance 2 (A complex mixture which includes alkyl naphthalenes, alkyl biphenyls, and alkyl benzenes + high nonene alkylation. batch no S001218) a NOEC could be calculated of 10 mg/L test substance.
The results obtained indicate that there is a slightly higher toxicity observed for test substance 2. As the results of this range finding test are considered to be valid and because both test substances are very similar in their fate during the daphnia test, the conclusion as obtained from this test is considered sufficient to select the more toxic substance as the worst-case representative in the hazard assessment of both substances if the higher toxicity observed for substance 2 is confirmed in an algae range finding test.
Reference
Test substance 1
Concentration (mg/L) |
Batches/Total |
Number of mobile animals |
||
0 hours |
24 hours |
48 hours |
||
Control
|
Batch I Batch II Total |
5 5 10 |
5 5 10 |
5 5 10 |
0.01 |
Batch I Batch II Total |
5 5 10 |
5 5 10 |
5 5 10 |
0.1 |
Batch I Batch II Total |
5 5 10 |
5 5 10 |
5 5 10 |
1.0 |
Batch I Batch II Total |
5 5 10 |
5 5 10 |
5 5 10 |
10.0 |
Batch I Batch II Total |
5 5 10 |
5 5 10 |
5 5 10 |
100.0 |
Batch I Batch II Total |
5 5 10 |
5 5 10 |
5 5 10 |
Test substance 2
Concentration (mg/L) |
Batches/Total |
Number of mobile animals |
||
0 hours |
24 hours |
48 hours |
||
Control
|
Batch I Batch II Total |
5 5 10 |
5 5 10 |
5 5 10 |
0.01 |
Batch I Batch II Total |
5 5 10 |
5 5 10 |
5 5 10 |
0.1 |
Batch I Batch II Total |
5 5 10 |
5 5 10 |
5 5 10 |
1.0 |
Batch I Batch II Total |
5 5 10 |
5 5 10 |
5 5 10 |
10.0 |
Batch I Batch II Total |
5 5 10 |
5 5 10 |
5 5 10 |
100.0 |
Batch I Batch II Total |
5 5 10 |
5 5 10 |
5 3 8 |
Description of key information
The acute toxicity of Sodium diisobutylnaphthalenesulphonate to daphnia is read across from the structurally related substance naphthalenesulfonic acid, bis(1-methylethyl)-, methyl derivatives, sodium salts.
In addition acute daphnia rangefinding tests have been performed with two batches of Aromatic Hydrocarbons, C10-13, Reaction Products With Branched Nonene Sulphonated, sodium salt to evaluate which of two relatively similar alkylnaphthalene sulphonates is the more toxic substance. The results obtained indicate that there is a slightly higher toxicity observed for the batch containing a higher fraction of long alkyl chain derivative when compared to the batch with a lower content of long alkyl chain derivative.
Comparable EC50 are observed in the acute daphnia test with naphthalenesulfonic acid, bis(1-methylethyl)-, methyl derivatives, sodium salts and Aromatic Hydrocarbons, C10-13, Reaction Products With Branched Nonene Sulphonated, sodium salt as for both EC50 values are observed of >100 mg/L. The study with naphthalenesulfonic acid, bis(1-methylethyl)-, methyl derivatives, sodium salts is however a complete study and the result as observed for this substance is there use for read across to those alkylnaphthalenesulphonates for which the acute daphnia data are missing.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
In the acute immobilization test with Daphnia magna(STRAUS), the effect of the test item Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt was determined according to OECD 202 (2004).
The test item is a substance of unknown or variable composition (UVCB). Due to the complex nature of the test item for the purposes of the test, the test item was prepared following the protocol of water accommodated fraction (WAF). Since the water solubility of the test item is above the tested limit concentration, the test solution is considered to be true solution at loading level. Therefore, the limit loading will be considered as the actual test item concentration throughout this report.
The study was performed under static conditions over a period of 48 hours with one concentration of the test item prepared as a limit test item concentration of 100 mg/L. All concentrations were visually clear throughout the exposure period and showed no Tyndall effect.
The concentrations of the test item Naphthalenesulfonicacid, bis(1-methylethyl)-, methyl dervis., sodium salt were analytically verified via UPLC-DAD in fresh media at the start of the exposure (0 hours) and the end of the exposure (48 hours) in the limit concentration and the control. Recoveries of the test item were in the range of 99 % at the start and end of exposure.
The measured concentration of the test item was within±20% of the nominal concentration.
In view of substances labeled as UVCB, all terms related to concentration levels should be given as loading levels because partly dissolved compounds and mixtures cannot be related to concentrations. However, since the limit test item concentration is below the certified water solubility and analytical recoveries of the main constituents were within nominal range, the EC10 / 50 / 100- are therefore based on the nominal test item concentration.
Effect values |
Test duration [hours] |
Naphthalenesulfonic acid, bis(1-methylethyl)-, methyl dervis., sodium salt Nominal concentration of the test item [mg/L] |
EC10/ EC50/ EC100 |
24 |
> 100 |
48 |
> 100 |
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