Pyridine-2-carbonitrile

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

Group of 10 male and 10 female albino rats were exposed (whole body exposure) continuously for seven hours to air (at 24°C) enriched with 2-Cyanopyridine vapour.

GLP compliance:

no

Remarks:

predates GLP

Test type:

concentration x time method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Pyridine-2-carbonitrile

EC Number:

202-880-3

EC Name:

Pyridine-2-carbonitrile

Cas Number:

100-70-9

Molecular formula:

C6H4N2

IUPAC Name:

pyridine-2-carbonitrile

Test material form:

solid

Specific details on test material used for the study:

> 98% purity

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

10 male and 10 female Wistar rats, 125-143 g and 114-127 g (f), SPF quality, bred at Schering AG, were maintained on standard diet (Altromin R) and tap water ad libitum. They were kept under standard conditions in Macrolon cages type II, with perforated bottoms (1 animal per cage) in air-conditioned rooms (room temperature 21-23°C, relative humidity 42-65%) under controlled light (12 hours day/night rhythm). The animals were earmarked, randomised and subdivided into two parallel inhaltion chambers into two groups, each of 5 male and 5 female rats. The acclimatization period (before trial start) was 7 days. The animals were kept without food for 17 hours before the beginning of inhalation. The observation period lasted for 22 days (day of exposure = day 1).

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

clean air

Details on inhalation exposure:

All the rats were exposed for seven hours on day 1 to an atmosphere enriched with 2-Cyanopyridine.

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

1

Concentrations:

"saturated vapor", relative humidity of 12% and temperature of 24°C were measure in the chambers.

No. of animals per sex per dose:

10 male, 10 female

Control animals:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC0

Effect level:

other: saturated vapour

Based on:

test mat.

Exp. duration:

7 h

Remarks on result:

other: quantitative result not determined

Mortality:

No mortality during 22 day observation period.

Clinical signs:

other: No abnormality during the 7 hour exposure period. 2/20 rats exhibited temporary conjunctivitis that fully resolved by day 19.

Body weight:

Mean weight (male, chamber 1) = 128 g start of study; 259 g at day 22
Mean weight (female, chamber 1) = 122 g start of study; 195 g at day 22
Mean weight (male, chamber 2) = 135 g start of study; 292 g at day 22
Mean weight (female, chamber 2) = 117 g start of study; 194 g at day 22

Gross pathology:

No treatment-related adverse effects found

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Lethal concentration (LC50) of 2 -Cyanopyridine in rats is higher than saturated vapour concentration at 24°C.

Executive summary:

Lethal concentration (LC50) of 2 -Cyanopyridine in rats is higher than saturated vapour concentration at 24°C.