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EC number: 207-005-9
CAS number: 421-50-1
1,1,1-trifluoroacetone was checked for colour interference in aqueous
conditions and possible direct MTT reduction by adding the test item to
MTT medium. Because the solutions did not turn blue/purple nor a
blue/purple precipitate was observed it was concluded that
1,1,1-trifluoroacetone did not interfere with the MTT endpoint.
The mean absorption at 570 nm measured after treatment with
1,1,1-trifluoroacetone and controls are presented in the following
Table. The individual OD570 measurements are presented in an other Table
Mean absorption in the in vitro skin corrosion test with
SD= Standard deviation
Duplicate exposures are indicated by A and B
In this table the values are corrected for background absorption
(0.0420). Isopropanol was used to measure the background absorption.
The following Table shows the mean tissue viability obtained after
3-minute and 1-hour treatments with 1,1,1-trifluoroacetone compared to
the negative control tissues.
Viability, percentage of control
1 -hour application
Viability, percentage of control
100 / SD: 3.8
100 / SD: 10.3
90 / SD: 1.6
7 / SD: 21.4
11 / SD: 13.9
11 / SD: 20.6
Skin corrosion is expressed as the remaining cell viability after
exposure to the test item.
Historical control data for in vitro skin corrosion studies
3 -minute treatment
1 -hour treatment
n= Number of observations
The above mentioned historical control data range of the controls were
obtained by collecting all data over the period of December 2012 to
The assessment of the corrosive potential to skin of
1,1,1-trifluoroacetone was carried out, under GLP compliance, using an
in vitro skin corrosive test based on the guidelines described in: OECD
No. 431 (adopted 29 July 2016) and EU Method B.40 BIS.
The test consisted of topical application of 1,1,1-trifluoroacetone (
400 µl; excess amount, every 15 minutes for the 1 -hour exposure) on the
skin tissue for 3-minute and 1-hour. After exposure the skin tissue was
thoroughly rinsed to remove the test item followed by immediate
determination of the cytotoxic (corrosive) effect.
Cytotoxicity is expressed as the reduction of mitochondrial
dehydrogenase activity measured by formazan production from
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) at
the end of the treatment.
The relative mean tissue viability obtained after the 3-minute and
1-hour treatments with 1,1,1-trifluoroacetone compared to the negative
control tissues was 90% and 7% respectively.
Because the mean relative tissue viability for 1,1,1-trifluoroacetone
was not below 50% after 3 minutes treatment but was below 15% after 1
hour treatment, 1,1,1-trifluoroacetone is considered to be corrosive.
The substance should be classified as corrosive to skin subcategories 1B
and 1C according to UN GHS criteria.
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