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EC number: 207-005-9
CAS number: 421-50-1
The objective of this study was to determine the potential of
1,1,1-trifluoroacetone (CAS RN 421-50-1) and/or its metabolites to
induce reverse mutations at the histidine locus in several strains of Salmonellatyphimurium
(S. typhimurium; TA98, TA100, TA1535, and TA1537), and at the
tryptophan locus of Escherichiacoli (E. coli) strain WP2uvrAin
the presence or absence of an exogenous mammalian metabolic activation
The test was performed in two independent experiments, at first a direct
plate assay was performed and secondly a pre-incubation assay. The study
procedures described in this report were based on the most recent OECD
and EC guidelines. The vehicle of the test item was dimethyl
In the dose-range finding study, the test item was initially
tested up to concentrations of 5000 µg/plate in the strains TA100 and WP2uvrA
in the direct plate assay. In the first mutation experiment, the
test item was tested up to concentrations of 5000 µg/plate in the
strains TA1535, TA1537 and TA98.
In the second mutation experiment, the test item was tested up to
concentrations of 5000 µg/plate in the tester strains TA1535, TA1537,
TA98, TA100 and WP2uvrA in the pre-incubation
assay. In all three experiments the test item did not precipitate on the
plates except in the first mutation experiment in tester strains TA1535
(absence of S9-mix) and TA1537 (presence of S9-mix) where precipitate
was observed at the highest dose level tested and in tester strain
TA1535 in the presence of S9-mix where precipitate was observed at dose
levels of 512 µg/plate and upwards. The bacterial background lawn was
not reduced at any of the concentrations tested and no biologically
relevant decrease in the number of revertants was observed.
The negative and strain-specific positive control values were within the
laboratory historical control data ranges indicating that the test
conditions were adequate and that the metabolic activation system
The test item did not induce a significant dose-related increase in the
number of revertant (His+) colonies in each of the four
tester strains (TA1535, TA1537, TA98 and TA100) and in the number of
revertant (Trp+) colonies in tester strain WP2uvrA
both in the absence and presence of S9-metabolic activation. These
results were confirmed in a follow-up experiment.
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