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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1978-10-27 to 1979-06-08
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
no
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: lot number 04078 (XAS-1467)
- Purity: 99.20 %
- Sample composition: t-BGE (99.20 %), t-butoxy-2-propanol (0.34 %), tri-isobutylene (0.14 %), t-butanol (0.10 %), unidentified (0.22 %)
- Physical state: clear liquid, stable during test conditions
- Solubility in water: 40 g/L at 25 °C
Analytical monitoring:
not specified
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: method of preparation not reported
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna Straus, water flea
- The daphnids used for testing were raised in the 1702 Building Fish Laboratory.

Test type:
static
Water media type:
other: declorinated Lake Huron water
Limit test:
yes
Total exposure duration:
48 h
Test temperature:
20 °C
Nominal and measured concentrations:
[mg/L]: 1.00, 1.80, 5.60, 10.00, 18.00 (see also Table 1 in Any other information on materials and methods incl. tables.)
Details on test conditions:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality, %Response; daily

In the daphnid toxicity test, water flea (Daphnia magna STRAUS) were exposed for 48 h at 20 °C. An initial range finding test was used to determine the concentration series to be used in the definitive test. Definitive tests were rerun until the mortality data met the conditions for statistical analysis; for the daphnids toxicity test there were four runs
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
other: No Adverse Effect Level
Effect conc.:
< 1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95%-C.I.: 10-31 mg/L
Duration:
48 h
Dose descriptor:
other: Partial Kill Level
Effect conc.:
1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95%-C.I.: 10-31 mg/L
Duration:
48 h
Dose descriptor:
other: 100 % Kill Level
Effect conc.:
> 7.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95%-C.I.: 10-31 mg/L
Duration:
48 h
Dose descriptor:
LC10
Effect conc.:
2.36 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95%-C.I.: 0.67-3.92 mg/L
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
14.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95%-C.I.: 10.3-31.3 mg/L
Duration:
48 h
Dose descriptor:
other: LC90
Effect conc.:
94.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95%-C.I.: 40.3-981.5 mg/L
Details on results:
- Behavioural abnormalities: no abnormalities were mentioned
- Observations on body length and weight: no abnormalities were mentioned

- Mortality of control:
- Other adverse effects control:
- Abnormal responses:
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
- Effect concentrations exceeding solubility of substance in test medium:
Reported statistics and error estimates:
LC values were calculated from the observed mortality data using a computer program of Finney's method of probit analysis.
Validity criteria fulfilled:
not specified
Conclusions:
The 48-hr-Static acute Daphnid Toxicity test of the test material showed moderate toxicity to daphnids. The 48-hr LC50 for daphnids (Daphnia magna Straus) was 14.92 mg/L (95%-C.I.: 10-31 mg/L).
Executive summary:

The 48–hr-acute toxicity of tert-butyl glycidyl ether to Daphnia magna Straus was studied under static conditions. Daphnids were exposed to tert-butyl glycidyl ether at nominal concentration of 1.00, 1.80, 5.60 10.00 and 18.00 mg/L for 48 hr.

Mortalilty was observed daily. 

The 48 – hr LC50 was 14.92 mg/L. 

Based on the results of this study, tert-butyl glycidyl ether would not be classified as toxic to Daphnia magna Straus in accordance with the CLP classification system. 

This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates. 

Results Synopsis

Test Organism Age (e.g. 1st instar):

Test Type: Static

              

- 48 hr LC10: 2.36 mg/L                     95% C.I.: 10-31 mg/L

- 48 hr LC 50: 14.92 mg/L                   95% C.I.: 10 -31 mg/L

- 48 hr LC 90:  94.25 mg/L                  95% C.I.:  10 -31 mg/L

- 48 hr NOAEL: < 1.0 mg/L

- 48 hr Partial Kill Level: 1.0 mg/L

- 48 hr 100 % Kill Level: > 7.2 mg/L

Endpoints effected: Mortality

Description of key information

In a 48-hr-static acute Daphnid Toxicity, t-BGE showed moderate toxicity to daphnids. The 48-hr LC50 for daphnids (Daphnia magna Straus) was 14.92 mg/L (95%-C.I.: 10-31 mg/L).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
14.92 mg/L

Additional information