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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1978-10-27 to 1979-06-08
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
no
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: lot number 04078 (XAS-1467)
- Purity: 99.20 %
- Sample composition: t-BGE (99.20 %), t-butoxy-2-propanol (0.34 %), tri-isobutylene (0.14 %), t-butanol (0.10 %), unidentified (0.22 %)
- Physical state: clear liquid, stable during test conditions
- Solubility in water: 40 g/L at 25 °C
Analytical monitoring:
not specified
Vehicle:
no
Details on test solutions:
Fathead minnows were exposed to the test chemical for 96 h.
Test organisms (species):
Pimephales promelas
Details on test organisms:
TEST ORGANISM
- Common name: fathead minnows, Pimephales promelas Rafinesque
- Source: were purchased from Aquatic Systems, Inc., in Ludington, Michigan. They had been held in the 1702 Building Fish Laboratory for one month before testing.
- Length at study initiation (length definition, mean, range and SD): average length of 2.8 cm
Test type:
static
Water media type:
other: dechlorinated lake Huron water
Limit test:
yes
Total exposure duration:
96 h
Test temperature:
12 °C
Nominal and measured concentrations:
[mg/L]: 144.00, 155.00, 167.00, 180.00, 194.00 (see also Table 1 in Any other information on materials and methods incl. tables.)
Details on test conditions:
In the fish toxicity tests, fathead minnows (Pimephales promelas Rafinesque) were exposed to the test chemical for 96 hours in 12 °C dechlorinated Lake Huron water.
TEST SYSTEM
- Test design: static
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Range finding study: Yes. An initial range finding test was used to determine the concentration series to be used in the definitive test. Definitive tests were rerun until the mortality data met the conditions for statistical analysis; for the fish toxicity test there were three runs.
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
other: No Adverse Effect Level
Effect conc.:
< 135 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
other: Partial Kill Level
Effect conc.:
155 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
other: 100 % Kill Level
Effect conc.:
> 194 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC10
Effect conc.:
152 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: (95%-C.I.: 135-160 mg/L)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
172 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: (95%-C.I.: 165-182 mg/L)
Duration:
96 h
Dose descriptor:
other: LC90
Effect conc.:
196 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: (95%-C.I.: 185-224 mg/L)
Details on results:
t-BGE showed a low toxicity to Pimephales promelas.
Reported statistics and error estimates:
LC values were calculated from the observed mortality data using a computer program of Finney's method of probit analysis.
Validity criteria fulfilled:
not specified
Conclusions:
The 96-hr-static acute fish test of the test material t-BGE showed low toxicity to fish. The 96-hour LC50 for fish (Pimephales promelas Rafinesque) was 172 mg/L (95%-C.I.: 165-182 mg/L). The 24- and 48-hour fish mortality data showed no kill and, therefore, could not be analyzed.
Executive summary:

In a 96 -h acute toxicity study, Pimephales promelas Rafinesque (fathead minnows) were exposed to tertiary butyl glycidyl ether (t-BGE) at nominal concentrations of 144.00, 155.00, 167.00, 180.00 and 194.00 mg/L under static conditions. The 96-h LC50was 172 mg/L.  The NOAEL value, based on mortality, was <135 mg/L, respectively. Sublethal effects were not mentioned. Based on the results of this study with P. promelas Rafinesque, t-BGE does not have to be classified as hazardous to fish in accordance with Section 4.1.2.6., Annex I of Regulation 1272/2008/EC.

This toxicity study is classified as acceptable and satisfies the guideline requirement for acute toxicity studie with fish.

 

Results Synopsis

 

Test organism size/age (mean wet weight or length): 2.8 cm

Test Type (Flowthrough, Static, Static Renewal): static

 

LC10: 152 mg/L                     95% C.I.:  135 -160 mg/L

LC50: 172 mg/L                     95% C.I.:  165 -182 mg/L

LC90: 196 mg/L                      95% C.I.:  185 -224 mg/L

NOAEL: <135 mg/L                  Probit Slope (Using log conc.): 23.28

 

Endpoint(s) Effected: Mortality

Description of key information

In a 96-hr-static acute fish test the test material t-BGE showed low toxicity to fish. The 96-hour LC50 for fish (Pimephales promelas Rafinesque) was 172 mg/L (95 %-C.I.: 165-182 mg/L).

Key value for chemical safety assessment

LC50 for freshwater fish:
172 mg/L

Additional information