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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The acute toxicity of Diurea 8 (in base grease) to the freshwater fish rainbow trout (Oncorhynchus mykiss) has been investigated and gave a 96-Hour LL50 (lethal loading rate) value of greater than 100 mg/L WAF. The No Observed Effect Loading Rate (NOELR) rate was 100 mg/L WAF.

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Additional information

A study was performed to assess the acute toxicity of Diurea 8 (in base grease) to rainbow trout (Oncorhynchus mykiss) (Harlan 2011). The method followed was designed to be compatible with the OECD guideline 203, "Fish, Acute Toxicity Test".

Following a preliminary range-finding test, fish were exposed, in two groups of seven, to a Water Accommodated Fraction (WAF), at a single nominal loading rate of 100 mg/L for a period of 96 hours at a temperature of approximately 14°C under semi-static test conditions. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the test until termination after 96 hours. Given that toxicity cannot be attributed to a single component or mixture of components but to the test item as a whole, the results were based on nominal loading rates only.

The 96-Hour LL50 (lethal loading rate) based on nominal loading rates was greater than 100 mg/L WAF. The No Observed Effect Loading Rate (NOELR) was 100 mg/l loading rate WAF. It was considered unnecessary and unrealistic to test at loading rates in excess of 100 mg/L.