[2-(1-ethoxyethoxy)ethyl]benzene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1974

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Pre-GLP study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1974

Report date:

1974

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

no

Remarks:

pre-GLP

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

1

Reference substance name:

[2-(1-ethoxyethoxy)ethyl]benzene

EC Number:

219-868-9

EC Name:

[2-(1-ethoxyethoxy)ethyl]benzene

Cas Number:

2556-10-7

Molecular formula:

C12H18O2

IUPAC Name:

[2-(1-ethoxyethoxy)ethyl]benzene

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Ace Animals
- Weight at study initiation: 235-287 g
- Fasting period before study: 16-20 hours
- Housing: 5/cage in suspended wire mesh cages. Bedding was placed beneath the cages.
- Diet: fresh Purina Rat Chow (Diet #5012), ad libitum, except 16-20 hours prior to dosing
- Water: ad libitum
- Acclimation period: at least 1 week

ENVIRONMENTAL CONDITIONS
- Photoperiod (hrs dark / hrs light): 12 / 12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Doses:

5000 mg/kg bw

No. of animals per sex per dose:

10 animals per dose

Control animals:

no

Details on study design:

- Frequency of observations: Animals were observed 3-4 hours post dosing and once daily thereafter for 14 days for mortality, toxicity and pharmacological effects.
- Necropsy of survivors performed: yes
- Other examinations performed: mortality, toxicity and pharmacological effects.

Results and discussion

Effect levels

Key result

Sex:

not specified

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

There were no mortalities.

Clinical signs:

other: none

Gross pathology:

none

Applicant's summary and conclusion

Interpretation of results:

other: Not acute toxic

Remarks:

Based on EU CLP criteria (EC 1272/2008 and its updates)

Conclusions:

The acute oral LD50 of Hyacinth body for rats was >5000 mg/kg bw. Based on the results of this study it is concluded that Hyacinth body is not acute orally toxic.

Executive summary:

An acute oral toxicity study with Hyacinth body was performed according to a method similar to OECD TG 401, and was rated Klimisch 2 as it was performed comparable to guideline study with acceptable restrictions and it was pre-GLP. In this study, 10 rats were administered Hyacinth body at dose level 5000 mg/kg bw. None of the animals died. The acute oral LD50 for rats was therefore set at >5000 mg/kg bw.