1-methyl-3-phenylpropylamine

Endpoint summary

Description of key information

Short term toxicity to aquatic invertebrates:

Short term toxicity of 1-methyl-3-phenylpropylamine (22374-89-6) on the growth and other biological activity of aquatic invertebrates is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine the effect of the structurally and functionally similar read across chemicals. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs. The stock solution 100 g/l was prepared by dissolving colourless liquid in acetone. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. Test conducted on different test concentration 0, 0, 6, 10, 17, 29 and 50 mg/l. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 15.5 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, indicates that the substance is likely to be hazardous to aquatic invertebrates and classified as per the CLP criteria. .

 

Similar study was performed for another structurally and functionally similar read across chemicals. Determination of the inhibition of the mobility of daphnids was carried out with the test substance. Test conducted according to the OECD Guideline 202. The stock solution 100 mg/l was prepared by dissolving colourless liquid in acetone. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 0, 5, 10, 20, 40, 80 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 20.5 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable thus it can be concluded that the test chemical was nontoxic and not classified as per the CLP classification criteria. 

 

In the third study for the structurally and functionally similar read across chemicals Determination of the inhibition of the mobility of daphnids was carried out with the test substance according to OECD Guideline 202. The stock solution 100 mg/l was prepared by dissolving transparent liquid in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the 0, 2.5, 5, 10, 20, 40 mg/l nominal concentration. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 16.6 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable in water and not stand in water for more time thus chemical may be consider to be nontoxic and not classified as per the CLP classification criteria.

 

Thus based on the overall studies for the structurally and functionally similar read across chemical 1-methyl-3-phenylpropylamine (22374-89-6), it can be concluded that the test chemical was classified as aquatic chronic 3 per the CLP classification criteria.  

Toxicity to aquatic algae and cyanobacteria:

Summarized result for the toxicity of chemical, on the growth of aquatic algae and cyanobacteria. Short term toxicity of 1-methyl-3-phenylpropylamine (22374-89-6) on the growth and other biological activity of aquatic algae is predicted on the basis of it experimentally structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals on the algae. The effect of test item was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga growth inhibition test. The test concentration chosen for the study were 6.25mg/L, 12.5mg/L, 25mg/L, 50 mg/L, 100mg/L and 200mg/L. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be >200mg/L. Thus, based on this value, test chemical can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.

 

Similar study was performed for another structurally and functionally similar read across chemicals. Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance. Test was conducted according to OECD Guideline 201. The stock solution 100 mg/l was prepared by dissolving colourless liquid in acetone. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 0, 5, 10, 20, 40, 80 mg/L. Effects on growth rate were observed for 72 hours. The median effective concentration (ErC50) for the test substance, in Desmodesmus subspicatus was determined to be 71.1 mg/L for growth rate effects. This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable thus it can be concluded that the chemical was nontoxic and not classified as per the CLP classification criteria.

 

Similarly third chemical was also supports the nontoxic nature of chemical which was structurally similar to the target chemical. Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the test substance according to OECD Guideline 201.The test substance was dissolved in OECD growth medium and tested at the concentrations 0, 5, 10, 20, 40 and 80 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (ErC50) for the test substance, in Desmodesmus subspicatus was determined to be 90.8 mg/L.

 Thus considering only the value from CLP Criteria for aquatic classification of the substance, it is concluded that test substance does not exhibits toxicity to aquatic algae (Desmodesmus subspicatus) and can be classified as aquatic chronic 3 as per the CLP classification criteria.

 

Thus based on the overall studies for the structurally and functionally similar read across chemical for 1-methyl-3-phenylpropylamine (22374-89-6), it can be concluded that the test chemical was nontoxic and not classified as per the CLP classification criteria.  

Additional information

Short term toxicity to aquatic invertebrates:

Short term toxicity of 1-methyl-3-phenylpropylamine (22374-89-6) on the growth and other biological activity of aquatic invertebrates is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine the effect of the structurally and functionally similar read across chemicals. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs. The stock solution 100 g/l was prepared by dissolving colourless liquid in acetone. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. Test conducted on different test concentration 0, 0, 6, 10, 17, 29 and 50 mg/l. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 15.5 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, indicates that the substance is likely to be hazardous to aquatic invertebrates and classified as per the CLP criteria.

 

Similar study was performed for another structurally and functionally similar read across chemicals. Determination of the inhibition of the mobility of daphnids was carried out with the test substance. Test conducted according to the OECD Guideline 202. The stock solution 100 mg/l was prepared by dissolving colourless liquid in acetone. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 0, 5, 10, 20, 40, 80 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 20.5 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable thus it can be concluded that the test chemical was nontoxic and not classified as per the CLP classification criteria. 

 

In the third study for the structurally and functionally similar read across chemicals Determination of the inhibition of the mobility of daphnids was carried out with the test substance according to OECD Guideline 202. The stock solution 100 mg/l was prepared by dissolving transparent liquid in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the 0, 2.5, 5, 10, 20, 40 mg/l nominal concentration. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 16.6 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable in water and not stand in water for more time thus chemical may be consider to be nontoxic and not classified as per the CLP classification criteria.

 

Thus based on the overall studies for the structurally and functionally similar read across chemical 1-methyl-3-phenylpropylamine (22374-89-6), it can be concluded that the test chemical was classified as aquatic chronic 3 per the CLP classification criteria.  

Toxicity to aquatic algae and cyanobacteria:

Summarized result for the toxicity of chemical, on the growth of aquatic algae and cyanobacteria. Short term toxicity of 1-methyl-3-phenylpropylamine (22374-89-6) on the growth and other biological activity of aquatic algae is predicted on the basis of it experimentally structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals on the algae. The effect of test item was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga growth inhibition test. The test concentration chosen for the study were 6.25mg/L, 12.5mg/L, 25mg/L, 50 mg/L, 100mg/L and 200mg/L. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be >200mg/L. Thus, based on this value, test chemical can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.

 

Similar study was performed for another structurally and functionally similar read across chemicals. Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance. Test was conducted according to OECD Guideline 201. The stock solution 100 mg/l was prepared by dissolving colourless liquid in acetone. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 0, 5, 10, 20, 40, 80 mg/L. Effects on growth rate were observed for 72 hours. The median effective concentration (ErC50) for the test substance, in Desmodesmus subspicatus was determined to be 71.1 mg/L for growth rate effects. This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic chronic 3 category as per the CLP criteria.

 

Similarly third chemical was also supports the nontoxic nature of chemical which was structurally similar to the target chemical. Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the test substance according to OECD Guideline 201.The test substance was dissolved in OECD growth medium and tested at the concentrations 0, 5, 10, 20, 40 and 80 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (ErC50) for the test substance, in Desmodesmus subspicatus was determined to be 90.8 mg/L. But as the substance was found to be readily bio-degradable in water compartment. Thus considering only the value from CLP Criteria for aquatic classification of the substance, it is concluded that test substance does not exhibits toxicity to aquatic algae (Desmodesmus subspicatus) and not classified as per the CLP classification criteria.

 

Thus based on the overall studies for the structurally and functionally similar read across chemical for 1-methyl-3-phenylpropylamine (22374-89-6), it can be concluded that the test chemical was nontoxic and not classified as per the CLP classification criteria.