1-methyl-3-phenylpropylamine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

experimental data of read across substances

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals.

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

WoE report is prepared based on short term toxicity to aquatic invertebrates study.

GLP compliance:

not specified

Specific details on test material used for the study:

Name: 1-methyl-3-phenylpropylamine
Mol. formula: C10H15N
Molecular Weight: 149.236 g/mole
InChI: 1S/C10H15N/c1-9(11)7-8-10-5-3-2-4-6-10/h2-6,9H,7-8,11H2,1H3
Smiles: c1(CC[C@@H](C)N)ccccc1

Analytical monitoring:

not specified

Vehicle:

yes

Remarks:

acetone

Details on test solutions:

1, 2. The stock solution 100 g/l was prepared by dissolving colourless liquid in acetone. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.
3.The stock solution 100 mg/l was prepared by dissolving transparent liquid in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Own breeding at University of Chemistry and Technology, Prague
- Age at study initiation (mean and range, SD): The animals used for the test shall be less than 24 h old and should not be first brood progeny
- Feeding during test: No feeding

ACCLIMATION - No data available
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

± 1 hr

Test temperature:

20±1°C

pH:

1. Test: 7.9 (changed to 7.8 during test)
Control: 7.8 (no change during test)
Control + acetone: 7.8 (changed to 7.9 during test)

2. Test: 7.8 (changed to 7.9 during test)
Control: 8 (Change to 8.1 during test)
Control + acetone: 7.9 did not change during the test

3.Test: 7.7 (no change during test)
Control: 7.8 (Change to 7.6 during test)

Dissolved oxygen:

1. higher than 7.6 mg/L at the end of test
2. higher than 7.3 mg/L at the end of test
3.higher than 7.8 mg/L at the end of test

Nominal and measured concentrations:

1. 0, 0, 6, 10, 17, 29 and 50 mg/l nominal test concentrations were used
2. 0, 0, 5, 10, 20, 40, 80 mg/l nominal concentration
3. 0, 2.5, 5, 10, 20, 40 mg/l nominal concentration

Details on test conditions:

1, 2, 3: TEST SYSTEM
- Test vessel: 50 ml glass vessel
- fill volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.
As an example, the preparation of dilution water meeting the requirements is described below.
Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.

Prepare the solutions specified below:
- Calcium chloride solution: Dissolve 117.6 g of calcium chloride dihydrate (CaCl2.2H2O) in water (4.2) and make up to 1 l with water (4.2).
- Magnesium sulfate solution: Dissolve 49.3 g of magnesium sulfate heptahydrate (MgSO4.7H2O) in water (4.2) and make up to 1 l with water (4.2).
- Sodium bicarbonate solution: Dissolve 25.9 g of sodium bicarbonate (NaHCO3) in water (4.2) and make up to 1 l with water (4.2).
- Potassium chloride solution: Dissolve 2.3 g of potassium chloride (KCI) in water (4.2) and make up to 1 l with water (4.2).

Mixing
Mix 2.5 ml of each of the four solutions and make up to 1 l with water.
The dilution water shall be aerated until the dissolved oxygen concentration has reached saturation and the pH has stabilized. If necessary, adjust the pH to 7.8 ± 0.5 by adding sodium hydroxide (NaOH) solution or hydrochloric acid (HCI). The dilution water prepared in this way shall not be further aerated before use.

- Sodium hydroxide solution, e.g. [NaOH] : 1 mol/l.
- Hydrochloric acid, e.g. [HCl] : 1 mol/l.

Reference substance:
Dissolve 600 mg of potassium dichromate (K2Cr2O7) in water and make up to 1 l with water (4.2).

OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment done
- Photoperiod: No - Darkness
- Light intensity:

CALCULATION:
EC50 was calculated using non linear regression by the software Prism 4.0

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7)

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

15.5 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95 % CI was 11.8 - 20.4 mg/l

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

20.5 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95 % CI was 16.4 - 25.7 mg/l

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

16.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95 % CI was 14.7-18.7 mg/l

Results with reference substance (positive control):

1, 2. Results with reference substance valid
- EC50: 0.73 mg/L (24 hours)

3. Results with reference substance valid
- EC50: 0.79 mg/L (24 hours)

Reported statistics and error estimates:

EC50 was calculated using non linear regression by the software Prism 4.0

Validity criteria fulfilled:

yes

Conclusions:

1. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 15.5 mg/L on the basis of mobiity inhibition effects in a 48 hour study.
2. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 20.5 mg/L on the basis of mobiity inhibition effects in a 48 hour study.
3. Based on the immobilisation of daphnia magna due to the exposure of test chemical , the EC50 was determine at 16.6 mg/l.

Executive summary:

Short term toxicity of 1-methyl-3-phenylpropylamine (22374-89-6) on the growth and other biological activity of aquatic invertebrates is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine the effect of the structurally and functionally similar read across chemicals on the invertebrates. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs. The stock solution 100 g/l was prepared by dissolving colourless liquid in acetone. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. Test conducted on different test concentration 0, 0, 6, 10, 17, 29 and 50 mg/l. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 15.5 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, indicates that the substance is likely to be hazardous to aquatic invertebrates and classified as per the CLP criteria. But as the chemical was readily biodegradable in water thus it concluded to be non-hazardous to aquatic invertebrates and cannot be classified as per the CLP criteria.

 

Similar study was performed for another structurally and functionally similar read across chemicals. Determination of the inhibition of the mobility of daphnids was carried out with the test substance. Test conducted according to the OECD Guideline 202. The stock solution 100 mg/l was prepared by dissolving colourless liquid in acetone. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 0, 5, 10, 20, 40, 80 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 20.5 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable thus it can be concluded that the test chemical was nontoxic and not classified as per the CLP classification criteria. 

 

In the third study for the structurally and functionally similar read across chemicals Determination of the inhibition of the mobility of daphnids was carried out with the test substance according to OECD Guideline 202. The stock solution 100 mg/l was prepared by dissolving transparent liquid in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the 0, 2.5, 5, 10, 20, 40 mg/l nominal concentration. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 16.6 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable in water and not stand in water for more time thus chemical may be consider to be nontoxic and not classified as per the CLP classification criteria.

 

Thus based on the overall studies for the structurally and functionally similar read across chemical 1-methyl-3-phenylpropylamine (22374-89-6), it can be concluded that the test chemical was nontoxic and not classified as per the CLP classification criteria.  

Description of key information

Short term toxicity of 1-methyl-3-phenylpropylamine (22374-89-6) on the growth and other biological activity of aquatic invertebrates is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine the effect of the structurally and functionally similar read across chemicals. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs. The stock solution 100 g/l was prepared by dissolving colourless liquid in acetone. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. Test conducted on different test concentration 0, 0, 6, 10, 17, 29 and 50 mg/l. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 15.5 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, indicates that the substance is likely to be hazardous to aquatic invertebrates and classified as per the CLP criteria and the chemical is biodegradable in water thus it concluded to be Aquatic Chronic 3 to aquatic invertebrates as per the CLP criteria.

 

Similar study was performed for another structurally and functionally similar read across chemicals. Determination of the inhibition of the mobility of daphnids was carried out with the test substance. Test conducted according to the OECD Guideline 202. The stock solution 100 mg/l was prepared by dissolving colourless liquid in acetone. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 0, 5, 10, 20, 40, 80 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 20.5 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable thus it can be concluded that the test chemical was nontoxic and not classified as per the CLP classification criteria. 

 

In the third study for the structurally and functionally similar read across chemicals Determination of the inhibition of the mobility of daphnids was carried out with the test substance according to OECD Guideline 202. The stock solution 100 mg/l was prepared by dissolving transparent liquid in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the 0, 2.5, 5, 10, 20, 40 mg/l nominal concentration. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 16.6 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable in water and not stand in water for more time thus chemical may be consider to be nontoxic and not classified as per the CLP classification criteria.

 

Thus based on the overall studies for the structurally and functionally similar read across chemical 1-methyl-3-phenylpropylamine (22374-89-6), it can be concluded that the test chemical was classified as aquatic chronic 3 per the CLP classification criteria.  

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

20.5 mg/L

Additional information

Short term toxicity of 1-methyl-3-phenylpropylamine (22374-89-6) on the growth and other biological activity of aquatic invertebrates is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine effect of the structurally and functionally similar read across chemicals on aquatic invertebrates. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs. The stock solution 100 g/l was prepared by dissolving colourless liquid in acetone. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. Test conducted on different test concentration 0, 0, 6, 10, 17, 29 and 50 mg/l. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 15.5 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, indicates that the substance is likely to be hazardous to aquatic invertebrates and classified as per the CLP criteria. But as the chemical was readily biodegradable in water thus it concluded to be non-hazardous to aquatic invertebrates and cannot be classified as per the CLP criteria.

 

Similar study was performed for another structurally and functionally similar read across chemicals. Determination of the inhibition of the mobility of daphnids was carried out with the test substance. Test conducted according to the OECD Guideline 202. The stock solution 100 mg/l was prepared by dissolving colourless liquid in acetone. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 0, 5, 10, 20, 40, 80 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 20.5 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable thus it can be concluded that the test chemical was nontoxic and not classified as per the CLP classification criteria. 

 

In the third study for the structurally and functionally similar read across chemicals Determination of the inhibition of the mobility of daphnids was carried out with the test substance according to OECD Guideline 202. The stock solution 100 mg/l was prepared by dissolving transparent liquid in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the 0, 2.5, 5, 10, 20, 40 mg/l nominal concentration. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 16.6 mg/L for immobilisation effects. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable in water and not stand in water for more time thus chemical may be consider to be nontoxic and not classified as per the CLP classification criteria.

 

Thus based on the overall studies for the structurally and functionally similar read across chemical 1-methyl-3-phenylpropylamine (22374-89-6), it can be concluded that the test chemical was classified as aquatic chronic 3 per the CLP classification criteria.