1-Naphthaleneethanamine, 7-methoxy-, hydrochloride (1:1)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

No analytical determination was performed

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

yes

Remarks:

No analytical determination was performed

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

604-144-0

EC Number:

604-144-0

Cas Number:

139525-77-2

Molecular formula:

C13H15NO.HCl

IUPAC Name:

604-144-0

Test material form:

solid: particulate/powder

Details on test material:

White powder

Specific details on test material used for the study:

Batch n°839411/006Purity : 100,0%Storage : room temperature (20 +/-5 °C), dry

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Variety : StrausStrain : BerlinSex : femaleAge: between 0 and 24 hoursOrigin : Umweltbundesamt BerlinArrival of strain : October 4th 2005

Study design

Test type:

static

Water media type:

freshwater

Remarks:

Deionised water with an enrichment of certain minerals (as demanded in the guideline)

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

24 and 48 hours

Test conditions

Test temperature:

23 - 23.2°C

pH:

7.1 - 7.8

Dissolved oxygen:

7.0 mg/L

Nominal and measured concentrations:

Nominal : 1/ 10/ 100 mg/L

Details on test conditions:

Before the study, there is a selection of daphnia. Replciates : Four vessels, each containing 20 mL test solution and five daphnia. Control : Four vessels, each containing 20 mL dilution water and five daphnia. Test vessels glass beakers, nominal volume 50 mL, tall shapeTemperature 20 +/- 2°C

Reference substance (positive control):

yes

Remarks:

K2Cr2O7

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

ca. 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 10 - < 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 - < 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

The 24h-EC50 of potassium dichromate was tested in a current reference test. The value was determined as 1.4 mg/L, lyong within the demanded range of 0.6 - 2.1 mg/L.

Reported statistics and error estimates:

All validity critera were met; For the estimation of the EC50 of the reference item, the fit showed sufficient statistical correspondence of the data with the dose-responsa equation

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In accordance the guideline OECD 202, the following results could be determined for the Mephtamin HCL :48h NOEC = 1 mg/L and 48h EC50 = 1 - 10 mg/L

Executive summary:

The experiment was performed using three concentrations ranging from 1 to 100 mg/L. Twenty daphnia were exposed to the test ite for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted.

In the treatment 100 mg/l, all daphnia were immobilised and 65% were immobilised in the concentration 10 mg/L after 48 hours. None of the animals were immobilised in the control.

The 24h-EC50 of potassium dichromate was tested in a current reference test. The value was determined as 1.4 mg/L, lyong within the demanded range of 0.6 - 2.1 mg/L.

The following results could be determined for the Mephtamin HCL :48h NOEC = 1 mg/L and 48h EC50 = 1 - 10 mg/L