1-Naphthaleneethanamine, 7-methoxy-, hydrochloride (1:1)

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

yes

Remarks:

no analytical determination of the content of the test item in the test solution was made. The composition of stock solution II must be stated as deviation

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Deviations:

yes

Remarks:

no analytical determination of the content of the test item in the test solution was made. The composition of stock solution II must be stated as deviation

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

604-144-0

EC Number:

604-144-0

Cas Number:

139525-77-2

Molecular formula:

C13H15NO.HCl

IUPAC Name:

604-144-0

Test material form:

solid: particulate/powder

Details on test material:

White powder

Specific details on test material used for the study:

Batch : 839411/006Purity : 100.9 %Expiry date : 10.02.2008Storage : room temperature (20 +/-5°C), dry

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Three treatments tested : 1/ 10/ 100 mg/L

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

Strain : ChodatFamily : ChlorophyceaaOrder : ChlorococcalesObtained in August 2007 from the Collection of Alga, institut fur Pflanzenphysiologie of Universitat Gottingen. The Alga are kept as stock culture on solid agar at 8°C.

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Post exposure observation period:

0, 24, 48, 72h

Test conditions

Test temperature:

26-29°C

pH:

7.5 to 8

Details on test conditions:

light intensity : 6500 - 6900 lux

Reference substance (positive control):

yes

Remarks:

Potassium Dichromate (CAS 7778-50-9)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC100

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: The test solution with the concentration of 100 mg/L turned brown and showed precipitation during the test. As in the concentration 100 mg/L, no algal growth wwas determined microscopically at the end of the test, inhibition was stated as 100%.

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 10 - < 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 1 - < 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 4

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 1 - < 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 5

Duration:

72 min

Dose descriptor:

LOEC

Effect conc.:

ca. 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

In all test concentrations, significant inhibition of the algal growth was determined. The test item changes its colour when getting in contact with the test medium. As the lowest test concentration (1 mg/L) could be evaluated properly, it can be statedn that the EC50 is higher than 1 mg/L but lower that 100 mg/L (and in the of EC50 of biomass and cell number lower than 10 mg/L).

Results with reference substance (positive control):

For the estimation of the EC50 of the reference item, the fits showed sufficient statistical correspondence of the data with the dose response-equation. The EC50 of potassium dichromate was tested in a current reference test. The values were withi the normal range of the laboratory

Reported statistics and error estimates:

T-test used

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The study conducted in accordance with OEC n°201 indicates; for the mephtamnin HCL ! 72h LOEC = 1mg/L - 72h EC50 (growth rate) = 10 -100 mg/L - 72h NOEC = not detectable.

Executive summary:

The study was performed using three concentrations ranging from 1 to 100 mg/L. The treatment were used to incubate the unicellular freshwater green alga Desmodesmus subspicatus for a period of 72 hours. The cell concentration of each replicate was determined by measuring the absorption of the cuvettes at 440 nm every 24 hours with a spectral photometer. With there measured values, the number of cells was calculated (linear correlation between cell concentration and adsorption given). Then the growth rate, the area under the growth curv and the yield were determined.

As the test item showed reaction with the test medium turing brown during the test, the highest test concentration couldn't be evaluated. In this concentration, no algal growth was visible microscopically ; therefore, the concentration 100 mg/L was stated as EC100. The EC50 of potassium dichromate was tested in a current reference test. The values were within the normal range of the laboratory.

The following results fo the tests item were determined : 72h LOEC = 1mg/L - 72h EC50 (growth rate) = 10 -100 mg/L - 72h NOEC = not detectable.