Reaction mass of 3-phenoxybenzyl (1R,3R)-2,2-dimethyl-3-(2-methylprop-1-en-1-yl)cyclopropanecarboxylate and 3-phenoxybenzyl (1R,3S)-2,2-dimethyl-3-(2-methylprop-1-en-1-yl)cyclopropanecarboxylate (4:1)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2006

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The concentration of the test substance was not stable during the test. The test item likely absorbed to the glass wall or the test organisms.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

The test concentration was measured at 0, 24 and 48 hours.
The daphnia were checked for immobilization after 24 and 48 hours.

Vehicle:

yes

Details on test solutions:

0, 1.0, 1.8, 3.2, 5.6 and 10 µg/L, applied dissolved in acetone.

Test organisms (species):

Daphnia magna

Details on test organisms:

6 to 24 hours old daphnia have been used. The strain was daphnia magna Straus.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

no

Hardness:

Reconstituted test water: analytical grade salts were dissolved to obtain water hardness of 2.5 mmol l-1.

Test temperature:

21.8 - 22.1 °C

pH:

The pH ranged from 7.05 - 7.81.

Dissolved oxygen:

The dissolved oxygen was between 5.8 and 6.5 mg/L.

Salinity:

not applicable

Nominal and measured concentrations:

Nominal: 0, 1.0, 1.8, 3.2, 5.6 and 10 µg/L.
Measured: Levels could not be detected in any of the samples, presumably due to sorption of the test substance to the glass walls or to the test organisms.

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

ca. 3.2 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

ca. 5.6 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

IC50

Effect conc.:

ca. 4.6 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 1.1 - 5.6

Results with reference substance (positive control):

Not appplicable.

Reported statistics and error estimates:

Reference is made to Appendix B of the study report.

Nominal test concentration	No. of daphnia tested	No. of immobilized daphnia		% of of immobilized daphnia
[ug l-1]		24h	48h	24h	48h
0 (control)	20	0	0	0	0
0 (solvent control)	20	0	0	0	0
1.0	20	0	0	0	0
1.8	20	0	4	0	20
3.2	20	0	4	0	20
5.6	20	5	14	25	70
10	20	8	20	40	100

Validity criteria fulfilled:

yes

Conclusions:

Despite some deficiencies, the results are in line with other available data on d-Phenothrin.
EC0 = 3.2 µg/L
EC10 = 5.6 µg/L
IC50 = 4.6 µg/L

Executive summary:

EC0 = 3.2 µg/L

EC10 = 5.6 µg/L

IC50 = 4.6 µg/L

Description of key information

The acute toxicity to daphnia magna upon exposure during 48 hours was investigated ina GLP compliant OECD 202 study. The immobilisation IC50 value was found being 4.6 µg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

4.6 µg/L