Registration Dossier
Registration Dossier
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EC number: 221-698-5 | CAS number: 3195-78-6
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The influence of the test substance on the growth of the freshwater algae Pseudokirchneriella subcapitata was assessed in a static dose response test according to OECD 201 (2006). The 72h-EC10 and the 72h-ErC50 were determined to be nominal greater than 120 mg/L.
Key value for chemical safety assessment
Additional information
The toxicity of the test substance to the freshwater alga Pseudokirchneriella subcapitata was determined according to the principles of OECD 201 (2006) and the Commission Regulation (EC) No 761/2009/C3 (2009) under GLP conditions. The aim of the study was to assess the effects on growth rate and yield over a period of 72 hours. The test item is a light yellow, clear liquid, has a purity of > 99 % and is miscible in water in any ratio (at 23°C and 20.0 + 0.5°C). The stock solution was freshly prepared with dilution water before the start of the exposure (at 0 hours) in a measuring flask and mixed thoroughly by inverting the measuring flask several times by hand. The limit concentration was visually clear throughout the exposure period. The study was conducted under static conditions with an initial cell density of 6153 cells/mL. The toxicity of a solution with a nominal concentration of 120 mg/L was assessed in a limit test. Six replicates were tested for the test item concentration and for the control, respectively. The environmental conditions were within the acceptable limits.
The limit test concentration of the test substance and the control were analytically verified by LC-MS/MS at the start and the end of the exposure. The measured concentrations of the test substance at the start of the exposure were 113 % of the nominal test item concentration. At the end of the exposure, the measured concentrations were 97 % of the nominal test item concentration. The 72h-EC10 and the 72h-ErC50 were determined to be nominal greater than 120 mg/L. All effect values are based on the nominal concentration of the test item.
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