Registration Dossier
Registration Dossier
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EC number: 221-698-5 | CAS number: 3195-78-6
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
In the semi-static immobilization test, young Daphnia magna STRAUSS (< 24 h old) were exposed to the test item for 48 hours according to OECD 202 (2004). The EC50 value was determined to be nominal greater than 120 mg test item/L.
Key value for chemical safety assessment
Additional information
In the acute immobilization test (BASF SE, 50E0462/14X444, 2015) with Daphnia magna (STRAUS), the effects of the test substance with a nominal loading of 120 mg test item/L and a control (0 mg test item/L) were determined according to OECD 202 (2004) and Council Regulation (EC) No. 440/2008/C2 (2008) under GLP conditions. The study encompassed 4 replicates per concentration and 1 replicate per control. The number of the organisms per vessel was 5. The mobility of the Daphnia was determined in a static 48-hour test by visual observation after 24 and 48 hours. The concentrations of the test item were analytically verified via LC-MS/MS at the start (0 hours) and at the end of the exposure (48 hours) in the limit concentration and the control. The measured concentration of the test item at the start of the exposure (0 h) was 100 % of the nominal concentration. At the end of the exposure (48 h), the measured concentration of the test item was 101 % of the nominal concentration. The measured test item concentration was within ± 20 % of the nominal concentration. This indicates that the test item concentration was successfully maintained for the duration of the test. A reference substance (potassium dichromate) was used as positive control. The EC50-value of the reference item potassium dichromate after 24 h was determined to be 2 mg/L. The EC50-value of the reference item is also within the recommended range of 0.6 - 2.1 mg/L according to OECD-Guideline 202. The water quality parameters of the dilution water (pH-value, dissolved oxygen concentration, temperature, conductivity and total hardness) were measured at the end of the exposure (48 hours) in all replicates of the limit concentration and the control. After 48 hours the EC50 value of the test item was determined to be nominal greater than 120 mg test item/L.
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