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Short-term toxicity to aquatic invertebrates

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A combined limit/range-finding test was performed on the test substance by exposing 20 daphnids per test group to a filtered solution of the substance prepared at a loading rate of 100 mg/l for 48h. No acute immobilisation was observed in the test species at the end of the exposure preiod. No detectable amount of the test substance was found in the filtered solution when analysed by an LC-MS technique validated for such measurement. The NOELR was assigned as 100 mg/l based on the nominal concentration.

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