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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: guideline study but no GLP defined.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
1,3-dichloro-4-nitrobenzene
EC Number:
210-248-3
EC Name:
1,3-dichloro-4-nitrobenzene
Cas Number:
611-06-3
Molecular formula:
C6H3Cl2NO2
IUPAC Name:
1,3-dichloro-4-nitrobenzene
Test material form:
other: solid
Details on test material:
IUCLID4 Test substance: as prescribed by 1.1 - 1.4

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: one eye of each rabbit served as control and remained untreated
Amount / concentration applied:
100 mg
Duration of treatment / exposure:
24 h
Observation period (in vivo):
7 d
Number of animals or in vitro replicates:
3
Details on study design:
24 hours affter application of test item into the conjunctival sac, the treated eye was rinsed with saline.

Results and discussion

In vivo

Results
Irritation parameter:
other: according to DRAIZE
Basis:
mean
Time point:
other: 24, 48 and 72 h
Score:
0
Max. score:
110
Reversibility:
fully reversible
Remarks on result:
other: not irritating
Other effects:
-

Any other information on results incl. tables

 Reading  1 h        24 h        48 h              72           
 Animal No.  19  20  21  19  20  21  19 20   21  19  20  21
 1. Cornea                        
 a) cloudiness  0  0  0  0  0  0  0  0  0  0  0  0
 b) surface  0  0  0  0  0  0  0  0  0  0  0  0
 a=A*B*5  0  0  0  0  0  0  0  0  0  0  0  0
 2. Iris  0  0  0  0  0  0  0  0  0  0  0  0
 b=A*5  0  0  0  0  0  0  0  0  0  0  0  0
 3.conjunctiva                        
 a) reddening  1  1  1  0  0  0  0  0  0  0  0  0
 b) swelling  1  1  1  0  0  0  0  0  0  0  0  0
 c) defluxion  1  1  1  0  0  0  0  0  0  0  0  0
 c=(A+B+C)*2  6  6  6  0  0  0  0  0  0  0  0  0
 Total= a+b+c  6  6  6  0  0  0  0  0  0  0  0  0

Primary irritation value: 0/9= 0.0

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information
Executive summary:

In an eye irritation study according to OECD 405 3 rabbits were treated with 0.1 g of the test substance. One eye of each test animal remained untreated and served as control. After 24 h the treated eye was flushed with with saline. The observation period was 7 d. According to DRAIZE the substance was found to be not irritating.