Chrome antimony titanium buff rutile

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1988-05-02 to 1988-05-06

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Remarks:

no analytical measurement of the test suspensions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German industrial standard test guideline DIN 38 412, part L15.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Chrome antimony titanium buff rutile

EC Number:

269-052-1

EC Name:

Chrome antimony titanium buff rutile

Cas Number:

68186-90-3

Molecular formula:

(Ti, Sb, Cr) O2

IUPAC Name:

manganese(4+) trititanium(4+) pentaantimony(3+) chromium(3+) nickel(2+) octadecaoxidandiide

Test material form:

solid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The doses were chosen based on a range finding study.
The product was added to the test water without any pretreatment.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: golden orfe
- Strain: Leuciscus idus (golden variety)
- Source: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation: 7.8 cm (range: 6.6 - 9.1)
- Weight at study initiation: 4.7 g (range: 3.1 - 6.7)
- Feeding during test: no
- Frequency: Withdrawal of food before exposure: 1 day

ACCLIMATION
- Acclimation conditions: oil-free aerated and charcoal filtered tap water, flow-through system
Water temperature: 21 °C
Acclimation to test water and test temperature: 3 days
- Health during acclimation (any mortality observed): Health 100 %; Mortality 0 %

HOUSING
- Housing water: charcoal filtered dechlorinated water in continuous flow-through
- Aeration: oilfree air
- Light regime: 16:8 hours light:dark
- Hardness: 2.5 mM
- Carbon hardness: 2.5 mM
- Oxygen content: > 60% of maximum saturation
- pH: 8.0
- Water temperature: 11 - 20 °C
- Duration of housing: about 6 months
- Medical treatment: Twice with 0.05 mg/L Malachite green chloride and once with 10 mg/L tetracycline hydrochloride
- Feeding: Growing feed F/B 50; Ssniff Spezialdiaeten GmbH
- Amount of feed: ad libitum
- Mortality during the last 2 weeks of housing: 0.6 %

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

Total hardness: 2.5 mmol/L

Test temperature:

21 °C

pH:

7.4 - 8.3

Dissolved oxygen:

6.9 - 8.1 mg/L

Nominal and measured concentrations:

Nominal concentrations: control, 5000 and 10000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: aquaria open
- Material, size, fill volume: glass, 30 x 22 x 24 cm, 10 L
- Aeration: aeration with oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 4.7 g fish /L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, part 11
- Ca/Mg ratio: 4 : 1
- Intervals of water quality measurement: 1, 24, 48, 72, and 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hour light:dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality, symptoms

Reference substance (positive control):

yes

Remarks:

Chloracetamid

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

dispersed

Basis for effect:

mortality (fish)

Details on results:

No adverse effects observed after 96 h.

Results with reference substance (positive control):

- Results with reference substance valid? yes
- LC50: ca. 32 mg/L (48 h)

Any other information on results incl. tables

Sublethal observations / clinical signs:

The test substance is insoluble; the test solution is coloured and cloudy.
Undissolved test substance is visible on the bottom of the aquaria. The results are based on nominal concentrations.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Chrome antimony titanium buff rutile is not acutely toxic to fish in a test according to the German industrial standard test guideline DIN 38 412, part L15 using golden orfe (Leuciscus idus) with suspensions prepared at nominal concentrations far exceeding its water solubility and the respective LC50 is estimated with > 10000 mg/L (nominal, dispersed). However, since no analytical measurement of the test suspensions was performed, the reliability of the study is rated RL3.