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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Easily elimated from water.

Key value for chemical safety assessment

Additional information

Several biodegradation studies are reported for 1,3,5 trioxane. Based on the results of these studies, it can be concluded that trioxane is inherently biodegradable. Trioxane was found not to be biodegradable in 28 days using a MITI test. Volatilization was found to be a significant mechanism of loss under aeration conditions. A standard Zahn-Wellens (OECD 302B) inherent biodegradation test suggested biodegradation was occurring but the relative contribution of volatilization was not determined. A modified Zahn-Wellens test was conducted using a closed system with sodium nitrate as the oxygen source. In this study, more than 90% of the DOC was removed in 18 days while only minimal loss of DOC was observed in controls without bacterial inoculum. This indicates that Trioxane is effectively removed via biodegradation from wastewater at a treatment plant.