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EC number: 200-579-1 | CAS number: 64-18-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1985
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Formic acid
- EC Number:
- 200-579-1
- EC Name:
- Formic acid
- Cas Number:
- 64-18-6
- Molecular formula:
- CH2O2
- IUPAC Name:
- formic acid
- Details on test material:
- - Name of test material (as cited in study report): Ameisensäure 99%
- Analytical purity: 99%
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Bor: WISW
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: F. Winkelmann, 4799 Borchen, Germany
- Weight at study initiation: males 126 g, females 117 g
- Fasting period before study: 16 h prior to dosing
- Housing: 1 to 5 rats per cage in Macrolon cages
- Diet: complete diet ad libitum
- Water: free access to tap water ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20
- Humidity (%): 65
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
-
MAXIMUM DOSE VOLUME APPLIED: 0.41 to 0.82 mL/kg bw
DOSAGE PREPARATION: undiluted
- Doses:
- 501, 631, 794, 1000 mg/kg bw
- No. of animals per sex per dose:
- 10
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: daily observations for clinical signs; body weight was recorded before treatment, and on days 1, 7, and 14 thereafter
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight - Statistics:
- The LD50 was calculated according to Litchfield and Wilcoxon (1949)
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 730 mg/kg bw
- 95% CL:
- 618 - 863
- Remarks on result:
- other: neat formic acid
- Mortality:
- Deaths occurred within 8 days after dosing; cf. tabulated detail information in the field “Remarks on results". The combined oral LD50 for male and female rats was 730 mg/kg bw.
- Clinical signs:
- other: Clinical signs were noted 30 minutes after dosing. Symptoms included unkempt fur, hunched posture, stagger, aggressiveness, dyspnea, sedation and ataxia, lateral and abdominal position, convulsions, bloody noses, blood in urine. At later times hypother
- Gross pathology:
- Dead animals: Hyperemia of the stomach and intestines. Mottled livers and kidneys.
Sacrificed animals: Hyperemia of the stomach. Mottled livers and discoloration of kidneys and pancreas
Any other information on results incl. tables
Mortality
The combined LD50 for male and female rats was 730 mg/kg bw.
=========================================================
Dose Mortality Death occurred
(mg/kg bw) (No. dead/exposed) after
male female
---------------------------------------------------------
501 0/5 1/5 within 1 hour
631 2/5 2/5 within 24 hours
794 1/5 5/5 within 8 days
1000 4/5 4/5 within 48 hours
=========================================================
Clinical signs
Clinical signs were noted 30 minutes after dosing. Symptoms included unkempt fur, hunched posture, stagger, aggressiveness, dyspnea, sedation and ataxia, lateral and abdominal position, convulsions, bloody noses, blood in urine. At later times hypothermia, body weight loss and pale limbs were additionally noted.
Symptoms subsided and were absent in all animals but one which showed symptoms until the end of the observation period.
Body weight gain was decreased in a dose-related manner
=========================================
Dose Mean body weight gains
(mg/kg bw) within 14 days post dosing
(g; males and females)
-----------------------------------------
501 56.1
631 45.9
794 28.3
1000 - 3.4
=========================================
Gross pathology
Dead animals:
Hyperemia of the stomach and intestines. Mottled livers and kidneys.
Sacrificed animals:
Hyperemia of the stomach. Mottled livers and discoloration of kidneys and pancreas.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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