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Diss Factsheets

Ecotoxicological information

Long-term toxicity to fish

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Administrative data

Endpoint:
fish early-life stage toxicity
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study followed basis scientific principles.

Data source

Reference
Reference Type:
publication
Title:
Acute and chronic toxicity of manganese to exposed and unexposed Rainbow and brown trout
Author:
Davies P and Brinkman S
Year:
1994
Bibliographic source:
Federal aid project F-243-1, Colorado Division of Wildlife, Fort Collins, Colorado

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Two sets of eyed rainbow trout eggs were used for testing, one set were acclimatised to manganese from the egg stage and one unacclimatised set.
Fish eggs were exposed to concentrations of the test material delivered via proportional diluted at a rate of two litres of test solution per cycle.

Under similar conditions, unexposed fish eggs were exposed to the same water conditions except no MnSO4 added.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Manganese sulphate
EC Number:
232-089-9
EC Name:
Manganese sulphate
Cas Number:
7785-87-7
Molecular formula:
H2O4S.Mn
IUPAC Name:
Manganese(II) sulphate

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
4 mo

Results and discussion

Effect concentrations
Duration:
4 mo
Dose descriptor:
NOEC
Effect conc.:
0.6 mg/L
Conc. based on:
other: Mn

Any other information on results incl. tables

Effect/no effect concentration and chronic value data show about a 2X increase in tolerance of exposed rainbow trout eggs

Table 1: Toxicity values

Effect/no effect conc. mg/L 

Chronic value mg/L

Acclimatised 

2.13 - 1.15 

1.57  

Unacclimatised 

1.04 - 0.60 

0.79

Table 2: Measured Mn concentration and mortality data

Exposure

1

2

3

4

5

6

7

 

Acclimatised group

Mn (mg/L)

6.61

5.08

3.74

2.13

1.15

0.61

<0.02

Std Dev

0.51

0.33

0.25

0.17

0.09

0.04

0.03

N

4

18

18

18

18

18

18

% mortality

100

95

90

35

0

0

0

 

Unacclimatised group

Mn (mg/L)

6.50

4.58

3.49

1.96

1.40

0.60

<0.02

Std Dev

0.35

0.36

0.18

0.12

0.12

0.14

0.03

N

5

5

18

18

18

18

18

% mortality

100

100

95

60

10.5

0

0

Table 3: Water quality data

 

Alkalinity mg/L

Hardness mg/L

pH

D.O. mg/L

Temp

°C

Mean

39.4

34.0

7.53

7.73

14.3

Water quality values were incorrectly reported in the paper. Corrected values are reported as an addendum in: Davies & Brinkman, (1995) Acute toxicity of manganese to Brown Trout (Salmo trutta) in hard water, Federal aid project #F-243, Colorado Division of Wildlife, Fort Collins, Colorado

Applicant's summary and conclusion

Validity criteria fulfilled:
no
Remarks:
The temperature was maintained at 15°C rather than 10°C. Hatching success of the control is not reported
Conclusions:
The study showed an increased tolerance of rainbow trout eggs chronically exposed to Mn2+ compared to unexposed eggs.