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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vivo

Currently viewing:

Administrative data

Endpoint:
in vivo mammalian cell study: DNA damage and/or repair
Remarks:
Type of genotoxicity: DNA damage and/or repair
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Peer reviewed data

Data source

Reference
Reference Type:
review article or handbook
Title:
Lack of influence of modulators of epoxide metabolism on the genotoxicity of trans-anethole in freshly isolated rat hepatocytes assessed with the unscheduled DNA synthesis assay
Author:
A.D. Marshall and J. Caldwell
Year:
1996
Bibliographic source:
Food and Chemical Toxicology 34 (1996) 337-345

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 486 (Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo)
GLP compliance:
not specified
Type of assay:
unscheduled DNA synthesis

Test material

Constituent 1
Chemical structure
Reference substance name:
(E)-anethole
EC Number:
224-052-0
EC Name:
(E)-anethole
Cas Number:
4180-23-8
Molecular formula:
C10H12O
IUPAC Name:
1-methoxy-4-prop-1-en-1-ylbenzene

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
trioctanonin (1 mL/kg bw)
Duration of treatment / exposure:
5h
Doses / concentrationsopen allclose all
Remarks:
Doses / Concentrations:
1E-6 - 1E-2 M
Basis:
analytical conc.
Remarks:
Doses / Concentrations:
0, 1, 125, 500 mg/kg
Basis:
nominal conc.
Control animals:
yes
Positive control(s):
2-acetylaminofluorene (2-AAF) and methyleugenol

Examinations

Tissues and cell types examined:
hepatocytes

Results and discussion

Test results
Sex:
male/female
Genotoxicity:
negative
Toxicity:
no effects
Vehicle controls validity:
valid
Negative controls validity:
valid
Positive controls validity:
valid

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): negative