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EC number: 224-052-0
CAS number: 4180-23-8
One valid experiment was performed using 5 concentrations ranging
from 1.0 to 22 mg/L nominal concentration. Ten daphnia per concentration
were exposed individually to the test item and the control for 21 days.
The offspring of the animals was counted three times a week.
Decrease in reproduction was observed in the two highest treatments,
ranging from 26 % to 98 %, compared to the control.The highest
concentration showed also clear mortality (80 %).
The content of the test item in the test solutions was determined
using GC/FID determination. The analysis of the test item concentrations
in the treatments before and after exposition showed an acceptable
stability of the test item during exposition. The geometric means of the
measured concentrationsin the fresh prepared and the old solutionswere
calculated. Their total mean was used for the evaluation of the
The test item was present in the solutions on day 2, but in very
low concentrations, because of adsorption or ingestion onto the algae
cells and daphnids. Therefore, one additional replicate per
concentration without daphnids and algae were prepared for the
analytical measurement. The geometric mean of day 0-2 was not used for
evaluation of biological results.
The following results were determined for the test itemSynthetic
NOEC = 1.05 mg/L
LOEC = 2.44 mg/L
EC50= 2.81 mg/L
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