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EC number: 224-052-0
CAS number: 4180-23-8
The effects of the test item on the metabolic activity of soil micro-organisms were determined according to OECD Guideline 216 (2000) over 28 days. The test item was applied via quartz sand with the test concentrations 1000 - 500 - 250 - 125 - 62.5 mg product/kg soil dry weight (factor 2). Untreated soil was tested as control under the same test conditions as the test item concentrations. Standard soil, moistened to nominally 45 % of its maximal water holding capacity, was used. Plastic boxes (volume 1.0 L) with perforated tops to enable gas exchange and filled with 400 g soil dry weight were used. The effects of the test item on the metabolic activity of the nitrogen-N formation rate (nitrate) were measured on the day of treatment (day 0) and after 7, 14 and 28 days. On day 28 the EC50 for nitrogen transformation in soil measured as nitrate was > 1000 mg/kg soil dry weight, the EC10 for nitrogen transformation in soil measured as nitrate was 710 mg/kg soil dry weight.
Based on results of an OECD 216 compliant study with the test item an EC10 (based on the Nitrate-N formation rate) of 710 mg/kg soil dw was determined as key value for the risk assessment.
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