Registration Dossier

Administrative data

Endpoint:
adsorption / desorption
Remarks:
other: absorption coefficient
Adequacy of study:
other information
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study conducted to GLP in compliance with agreed protocols with no deviations from standard test guidelines and/or minor methodological deficiences which do not affect the quality of the relevant results

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: Method C19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008
GLP compliance:
yes (incl. certificate)
Type of method:
other: HPLC screening method

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): INK BH11 M
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type: dark red powder
- Lot/batch No.: MB-1A
- Storage condition of test material: room temperature in the dark

Study design

Test temperature:
30 C

HPLC method

Details on study design: HPLC method:
EQUIPMENT
- Apparatus: HPLC System Agilent Technologies 1050, incorporating autosampler and work station
- Type: Column type Zorbax SB-CN 5u (250 x 4.6 mm id)
- Type, material and dimension of analytical (guard) column:
- Detection system:


MOBILE PHASES
- Type: Methanol:reverse osmosis water (55:45 v/v); adjusted to pH 7, with 0.1M sodium hydroxide
- Experiments with additives carried out on separate columns: yes / no
- pH: 7.0
- Solutes for dissolving test and reference substances:


DETERMINATION OF DEAD TIME
- Method: dead time determined by measuring the retention time of formamide (purity >/= 99.5%, 620 mg/L solution in mobile phase


REFERENCE SUBSTANCES
- Identity: acetanilide, phenol, atrazine, isoproturon, triadimenol, linuron, maphthalene, endosulfan-diol, fenthion, alpha-endosulfan, phenanthrene, diclofop-methyl, DDT



DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: injection volume 10 uL
- Quantity of reference substances: See Table 11.1
- Intervals of calibration:


REPETITIONS
- Number of determinations: 2


EVALUATION
- Calculation of capacity factors k': see tables 11.2 and 11.3
- Calculation of retention times: see tables 11.2 and 11.3
- Determination of the log Koc value:see tables 11.2 and 11.3

Results and discussion

Adsorption coefficientopen allclose all
Type:
Koc
Value:
< 17.8
Type:
log Koc
Value:
< 1.25

Results: HPLC method

Details on results (HPLC method):
Test material contained numerous salted sulphonic acid groups, teseting was carried out at pH 7, with test material in fully ionised state

Applicant's summary and conclusion