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EC number: 213-834-7 | CAS number: 1025-15-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08 Aug - 14 Dec 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Version / remarks:
- 1997
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 1,3,5-triallyl-1,3,5-triazine-2,4,6(1H,3H,5H)-trione
- EC Number:
- 213-834-7
- EC Name:
- 1,3,5-triallyl-1,3,5-triazine-2,4,6(1H,3H,5H)-trione
- Cas Number:
- 1025-15-6
- Molecular formula:
- C12H15N3O3
- IUPAC Name:
- tris(prop-2-en-1-yl)-1,3,5-triazinane-2,4,6-trione
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: HsdWin: NMRI
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: about 5 weeks
- Weight at study initiation: 22.0-29.7 g (males); 19.4-25.3 g (females)
- Assigned to test groups randomly: yes, by numbered index cards
- Fasting period before study: overnight
- Housing: five mice of the same sex were caged together in Makrolon-cage type III
- Diet: Altromin 1324 (Altromin International, Lage, Germany), ad libitum
- Water: tap water, ad libitum
- Acclimation period: 6 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 3 °C
- Humidity (%): 65-75%
- Air changes (per hr): 8
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: From: 08 Aug To: 14 Dec 2000
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- - Vehicle/solvent used: tap water
- Duration of treatment / exposure:
- not applicable
- Frequency of treatment:
- single application
- Post exposure period:
- 24 h (all dose groups including control group) and 48 h (additional 1500 mg/kg bw and control group)
Doses / concentrations
- Remarks:
- Doses / Concentrations:
500, 1000 and 1500 mg/kg bw (5, 10 and 15% w/w)
Basis:
actual ingested
- No. of animals per sex per dose:
- 5
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- Cyclophosphamide
- Route of administration: single application by gavage
- Doses / concentrations: 30 mg/kg bw (0.3% w/w)
- Vehicle: tap water
Examinations
- Tissues and cell types examined:
- Tissue: bone marrow
Cell type: bone marrow cells - Details of tissue and slide preparation:
- DETAILS OF SLIDE PREPARATION:
Cell suspension was transferred to a clean, dry slide, a smear prpared, and the slide left to air-dry. The slides were stained manually according to the technique of Pappenheim with May-Grünwald and Giemsa solutions.
METHOD OF ANALYSIS:
The slides were examined under light microscope. At high magnification a total of at least 2000 erythrocytes per animal were examined. Each erythrocyte scored was classified as polychromatic or mature and at least 2000 poly-chromatic erythrocytes were examined for the presence or absence of micronuclei. The frequencies of micronucleated cells per 2000 polychromatic erythrocytes were then calculated together with the ratio of polychromatic to normochromatic cells. - Evaluation criteria:
- A test substance is classified as mutagen if it induces a significant increase in the number of micronucleated PCEs for at least one time point. A test substance producing no significant increase in the number of micronucleated PCEs at any time point is considered as non-genotoxic in this system.
- Statistics:
- Mean number of NCEs and PCEs and the mean number of micronucleated PCEs were calculated for each sex and for both sexes combined together with the standard deviation. Assay data analysis was performed using the Mann-Whitney-U-test (P > 0.05).
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Remarks:
- no depression in bone marrow proliferation
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- RESULTS OF DEFINITIVE STUDY
- Induction of micronuclei (for Micronucleus assay): The mean occurence of micronucleated PCEs was 0.17% and 0.06% 24 h and 48 h after application, respectively. These values are in the range of the historical negative control (0.06% - 0.24% micronucleated PCEs). The positive control animals had an increase in 4.13% indicating a genotoxic effect. In the test substance groups the frequency of detected micronuclei was in the range of the historical negative control in all doses and at any preparation time after application of the test substance. In the female animals 24h after application, there was a very slight dose-dependent increase in the frequency of micronuclei (500 mg/kg bw: 0.01%, 1000 mg/kg bw: 0.12%, 1500 mg/kg bw: 0.15%) in comparison to the corresponding negative controls (0.09% PCEs with micronuclei). However, statistical analysis of the data revealed no significant increase of the micronuclei ratio per 2000 PCEs. The observed very slight increase, which is still in the range of the historical negative controls, was considered to be of no biological relevance and does not indicate a dose-dependent treatment-related effect.
- Ratio of PCE/NCE (for Micronucleus assay): The ratio of PCE to NCE for the test substance groups were from 0.8 to 1.0 and are not different to the respective vehicle control groups.
- Statistical evaluation (Mann-Whitney U-Test, p > 0.05): Dose levels of 500, 1000 and 1500 mg/kg bw caused no statistical significant enhancement (in a test for p < 0.05) in the frequency of micronuclei compared to the negative control at any preparation interval. Because only one male animal survived until terminal sacrifice, statistical evaluation of the male dose group 1500 mg/kg bw 48 h p. a. was not possible. Significant increases over the negative controls were seen in positive control group animals
Any other information on results incl. tables
Application of 1500 mg/kg bw of the test substance resulted in toxic symptoms like sedation and excitation with tremors in male and female animals 24 h p.a.. In the high dose group 1 male was found dead 24 h after application and 3 males and 2 females were found dead 48 h after application. Animals of the high dose group showed body weight reduction of up to 15.5%. All other animals survived to scheduled termination. In the low and middle dose groups body weight gain was positive but in comparison to the negative control animals, the body weight gain was reduced.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
Under the conditions of this micronucleus assay, there was no evidence of an aneugenic or clastogenic effect of the test substance leading to micronucleus formation in polychromatic erythrocytes of treated mice 24 h or 48 h after oral administration up to 1500 mg/kg bw.
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