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EC number: 941-809-7
CAS number: -
The results of the MTT assay for negative and positive controls are
shown in Table 1. Optical density for negative control (PBS) was above
0.6 and the standard deviation value of the percentage of viability was
lower than 18 %. The tissue viability for the positive control (5 % SDS)
was below 40 %, with the standard deviation value lower than 18 %.
Therefore, the acceptance criteria were met for this experiment.
No interaction with MTT: no risk of false negatives.
The coloring potential of test-substances
There was no change to the colour of the tissues and no perturbation the
For each treated tissue, the tissue viability was expressed as a
percentage of the mean negative control. The tissue viability data
obtained with the MTT assay for test substances are presented in Table
1. In every case, the relative viabilities of tissues exposed to test
substances were above 50 % for neat concentration.
Table 1: summary results and classification
Viability mean (%)
Negative control (PBS)
Positive control (SDS 5%)
In an in vitro skin irritation study performed in accordance with
OECD draft proposal for a new guideline “In vitro Skin Irritation:
Reconstructed Human Epidermis (RhE) Test Method”, the test substance
“Sophorolipids: fermentation products of glucose and fatty acids,
C18-unsatd., esters with glycerol with yeast Candida Bombicola,
partially hydrolysed” was applied neat to reconstructed human epidermis
for an exposure period of 15 minutes. The number of replicate tissues
was three. After 15 minutes exposure the tissues were washed with
phosphate buffered saline. Subsequently the tissue constructs were
incubated for 42 h at 37 °C. Afterwards, tissues were placed in MTT
working solution and incubated at 37 °C, 5 % CO2, for
approximately 3 h. MTT was reduced by viable cells into blue formazan
crystals which were extracted and transferred for 570-nm optical density
reading in duplicate. Relative cell viability for each test compound was
calculated as the OD570 ratio to negative control.
The positive (5 % SDS) and negative (PBS) control gave responses that
were within the acceptance criteria and as such demonstrated the
validity of the study.
The relative mean tissue viability obtained after 15 minutes treatment
with the test substance compared to the negative control tissues was
113.2 %. Since the mean relative tissue viability for the test substance
was above 50 %,the test substance “Sophorolipids: fermentation
products of glucose and fatty acids, C18-unsatd., esters with glycerol
with yeast Candida Bombicola, partially hydrolysed” is identified to be
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