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EC number: 941-809-7
CAS number: -
Before the measurements the test item was a white solid. After the
test the samples looked clear and slightly beige. Also, inside the
capillaries the test item was nothomogeneouslydistributed
but some gaps (possibly due to gas generation) could be observed.
Melting temperature [°C]
In general, it had to be concluded that no melting occurred but
probably decomposition. This was based on the following point:
The melting/freezing temperature of the substance was determined
according to OECD guideline no. 102 [adopted on 27 July 1995] and EU
test method A.1 [Council Regulation (EC) No 440/2008] with the capillary
method. No melting point could be observed in the tested temperature
range; probably decomposition of the substance occurred.
A preliminary thermogravimetric measurement was performed over a
temperature range from room temperature to approx. 600 °C. Several mass
loss steps were registered with a total mass loss of 77.2 %, including
the mass loss during initial purging with nitrogen (0.4 %). The residue
was probably not pure inorganic but also organic material.
The course of the thermographic curve indicates no melting but
decomposition of the test item.
The residue looked black and foamy, coming through the hole of the
Two DSC measurements were performed in the temperature range from
20 – 250 °C, since the thermogravimetric test had shown evidence for
decomposition starting at approx. 150 °C. The course of the heat flow
curves confirmed the results of the thermogravimetric measurement. No
sharp endothermic peak indicating a melting point could be observed.
Reweighing after the measurement showed that the sample had lost
approx. 11.0 / 12.2 % of its mass. The remaining residues looked
dark-brown and foamy, coming through the hole of the crucible.
The melting temperature of the test item was determined by differential
scanning calorimetry according to OECD Test Guideline 102 [adopted on 27
July 1995] and EU test method A.1 “Melting/freezing temperature”,
Council Regulation (EC) No 440/2008 of 30 May 2008 at atmospheric
The course of the thermographic curve indicated no melting point but
decomposition of the test item. The residue looked black and foamy,
coming through the hole of the crucible. A preliminary thermogravimetric
test had shown evidence for decomposition starting at approx. 150 °C.
No melting point could be observed until decomposition started at
approximately 150 °C.
The melting point of Sophorolipids was determined by two different
methods, i.e. by the capillary method as well as by the DSC method. In
both cases no melting point could be observed. Instead, decomposition at
a temperature of ca. 150 °C occurred as observed in the DSC measurement.
In conclusion, no melting point could be observed until decomposition
started at approximately 150 °C.
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