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EC number: 941-809-7
CAS number: -
The test item is considered as being readily biodegradable.
Testing of hydrolysis and adsorption/desorption behaviour was not
conducted because the test item is readily biodegradable and has a
The biodegradability of Sophorolipids was investigated in three
a.OECD guideline 301F
The biodegradation of the test substance was investigated over a 28 day
period in a manometric Respirometry Test according to OECD Guideline 301
F (1992). The test medium was inoculated with microorganisms from a
digester of a sewage treatment plant mainly fed with municipal
The rate of degradation was monitored by measuring the quantity of
oxygen required to maintain a constant gas volume in the respirometer
flasks over 28 days.
Sodium benzoate was used as a degradable reference substance at a
concentration of 100 mg/L, along with a toxicity control at 100 mg/L
test substance and 100 mg/L sodium benzoate. The biodegradation of the
test substance was found to be at mean 69 % with a standard deviation of
0.8 % for a concentration of 100 mg test substance/L and at mean 85 %
with a standard deviation of 5.4 % for a concentration of 60 mg test
item/L. For a concentration of 100 mg test substance/L biodegradation
within the 10-day-window was found to be 61 %.
For a concentration of 60 mg test substance/L biodegradation within the
10-day-window was found to be 75 %.
The degradation of of the reference substance sodium benzoate had
reached 83 % within the first 14 days.
According to the guideline, the test item is considered as beingreadily
biodegradableunder the test conditions.
b. OECD guideline 311
The anaerobic biodegradation of the test substance was determined in a
test according to OECD guideline 311 (2006) and EN ISO 11734 (1998).
Biodegradation was monitored by continuous measurement of the gas
developed until the end of the test. Anaerobic sludge was used as
After 61 days a degradation of 110.7 % was observed. The degradation
exceeding 100 % might be explained by the following: In the test flasks
bacterial growth was enhanced by the well biodegradable test substance.
After digestion of the test substance the biomass started
self-digestion. Self-digestion of the previously increased biomass in
the test flasks might very well be higher than the control flasks.
Except for the degradation of the positive control, the validity
criteria were met. As the test item was readily and completely
biodegradable under test conditions, this deviation from the guideline
can be stated as uncritical.
Therefore, the test item is stated to bebiodegradable under anaerobic
c. Read-across study, OECD 301C test
The biodegradation of the test substance was determined in an OECD 301C
test (modified MITI test). The degradation was measured by means of
oxygen uptake. After 28 days more than 99 % of the test substance was
degraded, meeting the 10 day window requirement. Under the conditions of
the test the test substance wasreadily biodegradable.
The test item Sophorolipids was readily biodegradable in both an OECD
guideline 301F and an OECD guideline 311 test.
A supporting study based on a read-across approach is available which
gave comparable results.
Testing is not required. According
to the REACH Regulation, Annex VIII, column 2 the study does not need to
be conducted if the substance is readily biodegradable. Handbook
data describe that the test substance hydrolyses under to both acid and
alkaline conditions. Transformation products are sophorose and
deacetyled free acids.
Adsorption / desorption
An adsorption/desorption study was not conducted due to LogKow < 3 and
ready biodegradability of the test item. Instead, Koc was calculated
with EUSES 2.1.2 using the following parameters:
Molecular weight: 623 g/mol (molecular weight of main constituent based
on oleic acid as major fatty acid moiety for the acidic form with the
representative molecular formula of C 30 H 54 O 13)
Melting point: 150 °C
Vapour pressure: minimum (1E-06)
Water solubility: 0.2 g/L (according to critical micelle concentration
LogKow: 2.4, worst case value calculated from measurement of CMC and
solubility in octanol
Chemical class: default QSAR
Result Koc: 185
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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