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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
in vivo mammalian germ cell study: cytogenicity / chromosome aberration
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: According to the OECD SIDS document for sodium hydroxide (March 2002), a Klimisch 3 reliability score was assigned since an unsuitable test system is used.

Data source

Reference
Reference Type:
publication
Title:
Spermatocyte chromosome aberrations in two species of grasshoppers at two different ionic activities
Author:
Manna et al.
Year:
1966
Bibliographic source:
Nucleus, 9, 119-131

Materials and methods

Principles of method if other than guideline:
Species: grasshopper (Spathosternum prasiniferum) the grasshoppers were injected abdominally with 0.02 ml of a pH 9 NaOH solution and the testes were fixed after intervalls of 4, 18, and 24 h; no validated test
GLP compliance:
no
Type of assay:
chromosome aberration assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
no data

Test animals

Species:
other: grasshopper
Strain:
other: Spathosternum
Sex:
not specified

Administration / exposure

Route of administration:
intraperitoneal

Results and discussion

Test results
Sex:
not specified
Genotoxicity:
other: see additional information on results
Toxicity:
not specified
Vehicle controls validity:
not specified
Negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
Marked changes were observed in the spermatocyte chromosomes of the 24-h specimens; the frequency of chromatid and chromosome type breaks was 3.2% (18/564 cells examined); other abnormalities included multipolar spindels, asynchronous separation of chromosomes, distribution of chromosomes in small groups, extreme stickiness and clumping of chromosomes, and sticky bridges.

Applicant's summary and conclusion