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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
March 30 - April 01, 2010
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report date:
2010

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
1-(2,3-dichlorophenyl)piperazine hydrochloride
EC Number:
601-621-5
Cas Number:
119532-26-2
Molecular formula:
C10H13Cl3N2
IUPAC Name:
1-(2,3-dichlorophenyl)piperazine hydrochloride

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
140 -250 mg/L as CaCO3
Test temperature:
The room temperature was in the range 20.1 – 21.7°C during the test period.
pH:
The pH of the test medium after preparation was in the range 7.64 – 7.84 for test item solutions and 7.83 for control, therefore, the pH of the test medium was not adjusted before the start of the test.
Dissolved oxygen:
During the test period, the dissolved oxygen concentrations in the test medium and the control were always greater than 6.0 mg/L according to guideline that provided a minimum oxygen concentration of 3.0 mg/L.

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
24 h
Dose descriptor:
LOEC
Effect conc.:
3.16 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
24 h
Dose descriptor:
IC50
Effect conc.:
2.82 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: 2.19 - 3.63 mg/L
Duration:
48 h
Dose descriptor:
IC50
Effect conc.:
0.95 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: 0.68 - 1.32 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The obtained experimental results allowed to calculate the NOEC, the LOEC and the IC50 at 24 and 48h. The results are as follows:
24 h NOEC 1 mg/L
48 h NOEC 0.32 mg/L
24 h LOEC 3.16 mg/L
48 h LOEC 1 mg/L
24 h IC50 2.82 (95% confidence range: 2.19 – 3.63 mg/L)
48 h IC50 0.95 (95% confidence range: 0.68 – 1.32 mg/L)