Piperazine, 1-(2,3-dichlorophenyl)-, hydrochloride (1:1)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 10 - June 07, 2010

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPPTS 835.3110 (Ready Biodegradability)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1-(2,3-dichlorophenyl)piperazine hydrochloride

EC Number:

601-621-5

Cas Number:

119532-26-2

Molecular formula:

C10H13Cl3N2

IUPAC Name:

1-(2,3-dichlorophenyl)piperazine hydrochloride

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

The study was performed using aerobic activated sludge from ALSI wastewater treatment plant treating civil wastewater. The sludge was washed by ccentrifugation and the supernatant liquid phase decanted. A homogenized aliquot of the final sludge suspension was weighted, thereafter dried and the ratio of wet to dry weight was calculated as 3.4%.
Based on this ratio, calculated amounts of wet sludge were suspended in the test water to get a concentration equivalent to 3.0 g dry material/L. During holding, the sludge was aerated at room temperature in dark conditions. Before using, the sludge inoculums was diluted with test water to obtain a final concentration of 15 mg/L (dry weight).

Duration of test (contact time):

48 h

Initial test substance concentration

Initial conc.:

100 mg/L

Details on study design:

The assay on test item has been run in parallel with a ready biodegradable reference item, i.e. sodium benzoate, the inoculums control, the abiotic control containing the test item and a abioticizing agent (HgCl2) and the toxicity control, containing test item, reference compound and inoculum.
BOD flasks of 500 ml capacity, equipped with pressure sensors fitted on the bottle mouth, have been used as test units.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

Details on results:

The test item is considered to be stable at the test conditions for a time corresponding to the exposure time (28 days). Therefore, all reported results are related to the nominal concentration of the test item.
The ThODNH3 values were calculated to be equal to 1.67 mg O2/mg for reference item and 1.32 mg O2/mg for test item.
The oxygen consumption due to nitrification was measured at the begging and at the end of the test and the absence of nitrification was assessed.
The test complies with the validity criteria since the mean oxygen uptake in the inoculums control at the end of test period was 0 mg O2/L and there was not difference between duplicate values of test item degradation at the end of the period. Moreover, the reference item reached the biodegradation pass level (60%) on the fifth day (68.3%).
Moreover, the test item showed no inhibitory effects on the microorganism activity at the tested concentration, as demonstrated by the 25% of biodegradation exceeded in the toxicity control, already at the fourth day of test period.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

The test item has showed to be not ready biodegradable under the conditions applied in a Manometric Respirometry test, having showed degradation of 0% during the test period.