Registration Dossier

Ecotoxicological information

Toxicity to terrestrial arthropods

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

28 d NOEC (mortality, reproduction, Folsomia candida) = 500 mg/kg soil DW; ISO 11267, RL1, GLP; read-across from C20/22 ATQ

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil dwelling arthropods:
500 mg/kg soil dw

Additional information

No experimental data are available for C16-18 DMAPA amidoamine. However, studies are available with the source substance C20/22ATQ. For justification for read-across see endpoint summary "Terrestrial toxicity".

The effects of C20/22 ATQ on the reproduction of Folsomia candida in artificial soil were examined in a laboratory study. The study was carried out based on ISO 11267 (1999) and OECD Draft (2008).

The aim of the test was to determine the effects of the test item on the reproduction of Folsomia candida in artificial soil by cutaneous and alimentary uptake.The test item was incorporated once into artificial soil with the test item concentrations 62.5 – 125 - 250 – 500 – 1000 mg/kg DW. The control was artificial soil without the test or reference item. Additionally a solvent control was tested. Folsomia candida (12 days old) were placed into artificial soil. For the control and solvent control 8 replicates and for the test item 5 replicates were tested. In each replicate 10 juveniles were inserted. During exposure springtails were fed with granulated dry yeast. After 28 days, mortality and reproduction were assessed.


Test item

C20/22 ATQ

Test object

Folsomia candida


Artificial soil

Adult mortality


LOEC [mg/kg DW]



NOEC [mg/kg DW]



LD50/ EC50 [mg/kg DW]

                  > 1000

                   > 1000


The validity of the study was evaluated according to ISO 11267 (1999) and OECD Draft (2008). Control mortality should be 20%. In this study the mean control mortality (pooled controls) was 13.8%. The reproduction rate should be >/= 100 instars per control replicate. In this study the reproduction rate was between 245 and 670 instars per control replicate (pooled control).The coefficient of variation of the reproduction rate in the control should be 30.0%. In this study the coefficient of variation in the pooled controls was 25%.Thus, this study was considered to be valid.