Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 206-056-4 | CAS number: 298-07-7
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Within 28 days a degradation of 75 % for bis(2-ethylhexyl) hydrogen phosphate was determined according to the OECD Guideline 301 F. Therefore bis(2-ethylhexyl) hydrogen phosphate is considered as readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
To test for its biodegradability potential, bis(2-ethylhexyl) hydrogen phosphate was incubated for 28 days in continuously stirred 250 ml closed flask in the dark with an inoculum originally collected from a local, predominantly municipal wastewater treatment plant. The applied method was the OECD Guideline 301 F. In this assay, biodegradation was estimated by biological oxygen demand (BOD) over time. BOD was measured daily by a manometer. The incubation temperature was 20 +/-1°C. The concentration of innoculum was 30 mg /L and the one of the substance was 100 mg/L. Degradation was calculated by substracting the amount BOD in the negative (innoculum only) control from that in the substance or positive control at any given time point and divided by the chemical oxygen demand (COD) or thereotical oxygen demand (ThOD). The 28-day degradation was 75% forbis(2-ethylhexyl) hydrogen phosphate and 82% for the positive control (with reference substance).
A second test on ready biodegradability was conducted according to the national Japanese standard method comparable to the OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I)). After 14 days, the substance showed 0-17% degradation realting to BOD, and 0% degradation relating to the test substance concentration.
As recommended in Guidance R.7b (ECHA, 2012), in cases of conflicting test results, differences in stringency and the origin of the inoculum in order to check whether or not differences in the adaptation of the inoculum may be the reason are considered. The reported data are carefully checked and no differences in test design were found in both tests. The positive result in the well documented study for ready biodegradability is considered as indicative of rapid and ultimate degradation and the positive test result supersede the negative test result.
In conclusion the bis(2-ethylhexyl) hydrogen phosphate is considered as readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
