Basic copper zinc nitrate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

n.a.

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: no data concerning glp, purity

Justification for type of information:

Copper is the ion which will be interesest in toxiocolgy matters. So here will be make a data waiving form copper(I) oxide to copper hydroxide nitrate

Data source

Reference

Reference Type:

publication

Title:

the results of micorbial mutation for forty-three industrial chemicals

Author:

Shimizu et al.

Year:

1985

Bibliographic source:

Jpn. J. Ind. Health., Vol. 27, 1985

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

not specified

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Dicopper oxide

EC Number:

215-270-7

EC Name:

Dicopper oxide

Cas Number:

1317-39-1

Molecular formula:

Cu2O

IUPAC Name:

Copper(I)oxide

Constituent 2

Reference substance name:

copprt(I) oxide

IUPAC Name:

copprt(I) oxide

Details on test material:

purity: 90%

Method

Target gene:

n.a.

Species / strain

Species / strain 1

Species / strain / cell type:

other: S. typhimurium TA 1535, TA 1537, TA 1538, TA 98 and TA 100

Additional strain / cell type characteristics:

not specified

Species / strain 2

Species / strain / cell type:

E. coli WP2 uvr A

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

S9

Test concentrations with justification for top dose:

1, 5, 10, 50, 100, 500, 1000, 5000 µg/plate

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

yes

Positive controls:

yes

Positive control substance:

other: AF-2, ENNG, 9AC, 4NQO, B(a)P, 2AA

Results and discussion

Test results

Test results 1

Species / strain:

other: S. typhimurium TA 1535, TA 1537, TA 1538, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not determined

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Test results 2

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not determined

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Copper(I)-oxide is negative in the Ames Test with and without metabolic activation, so it can concluded that copper hydroxid nitrate is negative in the Ames Test too.