Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 266-235-8 | CAS number: 66204-44-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 September 2016 - 28 October 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- July 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- municipal wastewater treatment plant Breisgauer Bucht, 30 mg dry solids per litre
- Duration of test (contact time):
- 29 d
- Initial conc.:
- 10 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- The test was performed in accordance to OECD Guideline 301B (CO2 evolution Test).
18.1 mg of 3,3'-methylenebis(5-methyloxazolidine)N,Nmethylenbismorpholine/L, corresponding to 10 mg C/L were exposed in triplicate at 22.2 – 23.3°C in the dark in an inoculated culture medium.
Activated sewage sludge from the municipal wastewater treatment plant Breisgauer Bucht was used as inoculum. The dried solids concentration was 30 mg/L. The culture vessels were sealed and aerated with CO2-free air at a rate of 30 - 100 mL/min (1.6 – 5.5. bubbles/s) and stirred. The culture vessels were equipped with two subsequent absorber vessels.
CO2 analysis was done on day 0, 4, 7, 11, 14, 21, 28. At day 29, 2 mL of 4 M hydrochloric acid was added to drive out any inorganic carbonates, vessels resealed, aerated overnight and CO2 analysed. Controls with inoculated culture medium and standard material (sodium benzoate, 34.3 mg/L, corresponding to 20 mg C /L) were incubated in triplicate.
One toxicity control with 18.1 mg/L test item and 34.3 mg/L sodiumbenzoate, resulting 30 mg C/L, was performed. - Reference substance:
- other: sodium benzoate
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 89.8
- Sampling time:
- 29 d
- Details on results:
- The degradation extent of the test item at the end of the test was 89.8% (28 d after acidification,
mean of three replicates, see table°1 and fig°1). On day 4 the degradation extent of the test
item was already 66.1% (mean of three replicates, see table 1). The 10 d-window was therefore
met. - Results with reference substance:
- The reference compound sodium benzoate reached the pass levels for ready biodegradability
within 4 days. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The substance can be regarded as readily biodegradable, including the 10 days window criterion.
- Executive summary:
The degradation extent of the test substance at the end of the test was 89.8% (28 d after acidification, mean of three replicates, see table°1 and fig°1). On day 4 the degradation extent of the test item was already 66.1% (mean of three replicates, see table 1). The 10 d-window was therefore met. The degradation extent in the toxicity control was 84.3% within 14 days, the test substance had no inhibitory effect on the inoculum.
The inorganic carbon content of the mineral medium was below 5%. The total CO2 evolution of the inoculum blank was below 40 mg/L. Therefore; the validity critera can be considered as fulfilled. Studies on hydrolysis of the active substance indicate that at large dilutions the hydrolysis products are expected which are both readily biodegradable. Therefore, the substance as well as its hydrolysis products are expected to be extensively removed in biological treatment plants as well as in aquatic compartments. The constituents of the reaction product (active substance) are not expected to be volatile from aqueous solutions, therefore this removal mechanism could not influence the test results. It is not expected that the the low adsorptivity of the test substance influenced the CO2 evolution.
Reference
Description of key information
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.