Pyrolysis light oil from waste plastics

Eye irritation

Administrative data

Endpoint:

eye irritation: in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 492 (Reconstructed Human Cornea-like Epithelium (RhCE) Test Method for Identifying Chemicals Not Requiring Classification and Labelling for Eye Irritation or Serious Eye Damage)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Pyrolysis light oil from waste plastics

EC Number:

940-514-0

Molecular formula:

Not required for a UVCB-substance (mixture of multiple (>100) organic substances)

IUPAC Name:

Pyrolysis light oil from waste plastics

Test material form:

liquid: volatile

Test animals / tissue source

Species:

human

Test system

Vehicle:

other: Dulbecco`s Phosphate Buffered Saline

Controls:

yes, concurrent positive control

yes, concurrent negative control

Amount / concentration applied:

50 µL of the liquid test item were added to 1 mL of MTT solution in a 6-well plate and the mixture was incubated in the dark at 37 ± 1 °C, 5.0 ± 1 % CO2 and ≥ 95% relative humidity for 3 hours. 1 mL of MTT solution plus 50 µL of H2O demin. was used as negative control.
20 µL DPBS buffer and then incubated at 37 ± 1 °C, 5 ± 1 % CO2 and ≥ 95% relative hu-midity for 30 minutes. After that, 50 µL of the controls and the test item were applied in duplicate in one- minute- intervals

Duration of treatment / exposure:

28 minutes

Duration of post- treatment incubation (in vitro):

For post-treatment incubation, the tissues were incubated for 120 minutes

Number of animals or in vitro replicates:

two replicates for each tissue

Results and discussion

In vitro

Results

Irritation parameter:

other: %viability

Run / experiment:

1-3

Value:

> 0.8 - < 2.5

Vehicle controls validity:

valid

Negative controls validity:

valid

Positive controls validity:

valid

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Under the conditions of the test, NAPHTHA is considered non-eye irritant in the EpiOcu-larTM Eye Irritation Test.
After treatment with the test item, the mean value of relative tissue viability was reduced to 87.9 %. This value is above the threshold for eye irritation potential (≤ 60%).