Pyrolysis light oil from waste plastics

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2021

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

sexually immature young fish of the same age, length 1 – 2 cm

Test type:

static

Limit test:

yes

Total exposure duration:

96 h

Hardness:

35.6

Test temperature:

22.6 – 24.4 °C

pH:

7.5-7.8

Dissolved oxygen:

7.9-8.2 mg O2/L

Key result

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

> 37 mg/L

Conc. based on:

test mat.

Validity criteria fulfilled:

yes

Conclusions:

96h-LL50 > 37 mg/L

Executive summary:

One valid experiment was performed.
The toxicity towards Danio rerio was tested using a static test design.
Because the test item is a poorly soluble UVCB substance with constituents of different water solubility, in accordance with the OECD guidance document no. 23, the water-accommodated fraction (WAF) was prepared for the test.
The study was performed at the threshold concentration, the lowest concentration with significant inhibition against algae or daphnia (OECD guidance document, No. 126). The EC50 against daphnia was determined as 36.09 mg/L (LAUS study 19040301G201). The EC50 against algae (growth rate) was determined as 69.54 mg/L (LAUS study 19040301G301).
Therefore, this study was performed as a limit test at the concentration 37 mg/L.
The tested loading rate showed no mortality. No fish died or showed any signs of abnormal behavior in the control and the treatment.
At the start and at the end of the test, the content of the test item in the test solutions was estimated by determination of the total organic carbon (TOC) content in the test solutions using a carbon analyser.
Despite the poor solubility of the test item, the measured carbon concentration showed, that the test item was present in the test solution.
As the test item is a UVCB substance, according to OECD guidance document No. 23 (2019), the result is presented, based on the nominal WAF loading rate (see OECD guidance document no 23, § 177).
The following result for the test item NAPHTHA could be determined: 96h-LL50 > 37 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 37 mg/L