Registration Dossier
Registration Dossier
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EC number: 286-272-3 | CAS number: 85203-81-2
Specific investigations: other studies
Administrative data
- Endpoint:
- phototoxicity
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Secondary literature
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Reference Type:
- secondary source
- Title:
- OPINION CONCERNING ZINC OXIDE COLIPA n° S76
- Author:
- SCCNFP
- Year:
- 2 003
- Bibliographic source:
- SCCNFP/0649/03, final
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: EU Directive 67/548/EEC, Annex V, B41
- Principles of method if other than guideline:
- Neutral Red Uptake Phototoxicity Test in vitro
- GLP compliance:
- not specified
- Type of method:
- in vitro
Test material
- Reference substance name:
- Zinc oxide
- EC Number:
- 215-222-5
- EC Name:
- Zinc oxide
- Cas Number:
- 1314-13-2
- Molecular formula:
- ZnO
- IUPAC Name:
- oxozinc
- Details on test material:
- - Name of test material (as cited in study report): Uncoated and micronised ZnO.
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- Balb/c
Administration / exposure
- Route of administration:
- other: in medium
- Vehicle:
- other: EBSS/ 1% (v/v) ethanol
Doses / concentrations
- Remarks:
- Doses / Concentrations:1.0 to 100.0 mg/lBasis:nominal conc.
- Details on study design:
- Cell type : Balb/c 3T3 mouse fibroblasts, Clon 31Test vehicle : ZnO in EBSS/ 1% (v/v) ethanol solubilised/dispersed in a concentration range of 1.0 to 100.0 mg/l. Chlorpromazine in EBSSLight source : Metal-halide lamp with cut-off filters according to Spielmann et al. (40, 41). UVA 1667 mW/cm2 for 50 min (5.0 J/cm2), UVB: nondetectable.
Results and discussion
- Details on results:
- Both ZnO irradiated and unirradiated had similar cytotoxic concentration response curves. Both curves were similarly shaped with nearly identical NR50 values at approximately 9 mg/L. The ratio of [NR50 (-UV)/ NR50 + UV)] PIF equals 1, demonstrating that ZnO does not exhibit a phototoxic potential in this test.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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