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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2010
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report date:
2010

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-ethoxyethylamine
EC Number:
203-801-5
EC Name:
2-ethoxyethylamine
Cas Number:
110-76-9
Molecular formula:
C4H11NO
IUPAC Name:
2-ethoxyethylamine
Test material form:
other: lquid

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations:(nominial) 0, 3, 10, 30, 100 and 300 mg/l
- Sampling method: all testkoncentrations
- Sample storage conditions before analysis: Frozen at - 20 C right after sampling

Test solutions

Vehicle:
no
Details on test solutions:
- Concentrations:(nominial) 0, 3, 10, 30, 100 and 300 mg/l

Test organisms

Test organisms (species):
Skeletonema costatum
Details on test organisms:
TEST ORGANISM
- Source (laboratory, culture collection): DHI

Study design

Test type:
static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
19 ± 0.1 ºC
pH:
8.0 ± 0.2
Salinity:
26.9 ± 0.2 ‰
Nominal and measured concentrations:
Nominal concentrations: 0, 3, 10, 30, 100 and 300 mg/l
Analytical control over time of nominal concentrations
Time(hours) Nominal concentration (mg/l) Actual measured koncentration (mg/l)
0 300 270
0 100 79
0 30 21
0(Control) 0 <0.1
72 300 300
72 100 87
72 30 23
72(Control) 0 <0.1
Details on test conditions:


- Test vessels: 300 ml glass flasks with approx. 100 ml of test soulution

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater, filtered, collected in the North Sea near Hirtshals by Danmarks Fiskeriundersøgelser 26.10.2009
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol, CAS No. : 591-35-5

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
ca. 152 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
ca. 22 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
ca. 83 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- EC50: 1.55 mg/l
- Other:

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
EC50( h) of Ethanamine, 2-ethoxy, CAS No.110-76-9, is 152 mg/l for Skeletonema costatum