3,5-dichlorobenzoyl chloride

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Not reported

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: A non-GLP study conducted in accordance with generally accepted scientific principles, possibly with incomplete reporting or methodological deficiencies, which do not affect the quality of the relevant results.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1969

Report date:

1969

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3,5-dichlorobenzoyl chloride

EC Number:

220-813-6

EC Name:

3,5-dichlorobenzoyl chloride

Cas Number:

2905-62-6

Molecular formula:

C7H3Cl3O

IUPAC Name:

3,5-dichlorobenzoyl chloride

Details on test material:

RH 24,299
Appearance: white, granular solid with a faint odour which formed a liquid when heated to 30°C for the purpose of administration.

Test animals

Species:

rat

Strain:

other: Carworth (Sprague-Dawley derived)

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: adult
- Weight at study initiation: 139 - 191 g
- Fasting period before study: 3 - 4 hours
- Diet (e.g. ad libitum): Purina Laboratory Chow available ad libitum.
- Water (e.g. ad libitum): water available ad libitum.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: corn oil

Details on oral exposure:

10% weight per volume suspension in corn oil.

Doses:

100, 215, 464, 1000, 2150 and 4640 mg/kg/bw.

No. of animals per sex per dose:

5 male rats per dose level.

Control animals:

no

Details on study design:

Administration: After a three- to four-hour fasting period, the test material was administered by gastric intubation as a 10% weight-per-volume suspension in corn oil to groups of five male rats each at dosage level.

OBSERVATIONS AND RECORDS
Mortality and Toxic Effects: Recorded immediately after dosing; at one, four, and 24 hours; and once daily thereafter for a total of seven days.
Body Weights: Recorded at study initiation and again at termination

TERMINAL STUDIES
Surviving animals sacrificed by exsanguination following barbiturate (Diabutal) overdose after a seven-day observation period.
Gross Necropsy Performed on all animals which died during the study and on those sacrificed at termination.

Statistics:

Statistical Analysis: Mortality data analyzed by the method of Thompson, W. R., Bact. Rev. 11, 115-145, 1947, utilizing the tables of Horn, H. J., Biometrics 12, 311, 1956.

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

794 mg/kg bw

Based on:

test mat.

95% CL:

584 - 1 080

Remarks on result:

other: Confidence limits in mg/kg

Mortality:

See table 1.

There were no mortalities at the 100, 215 and 464 mg/kg dose levels.

At the 1000 mg/kg dose level, 3 animals died 24 hours after administration, followed by a further death during the 6 day observation period.

At the 2150 mg/kg dose level, 4 animals had died at the 4 hour observation, followed by a further death during the 6 day observation period.

At the 4640 mg/kg dose level, 4 animals had died at the 4 hour observation, followed by a further death by the end of the 24 hour period.

Clinical signs:

other: At the 100 and 215 mg/kg dose levels, no effects were observed. At the 464 mg/kg dose level, slight depression noted from one through 24 hours. At the 1000, 2150, and 4640 mg/kg dose levels, additional effects noted until death or termination included l

Gross pathology:

No gross pathology was observed at sacrifice.

Major necropsy findings at death: gross alterations at the 1000, 2150, and 4640 mg/kg levels consisted of pale spleen; dark red medulla; smooth and thin cardiac portion of stomach; smooth pyloric portion of stomach with dark pink areas; at the 4640 mg/kg dose level, thin, chalky-white walls of small intestine were observed.

Any other information on results incl. tables

Table 1 Cumulative Mortality Data

 

Values represent the number of animals dead per number of animals tested, cumulative.

 

Dose (mg/kg)	Time of Death
	Immediate	Hours			Days
		1	4	24	2 - 7
100	0/5	0/5	0/5	0/5	0/5
215	0/5	0/5	0/5	0/5	0/5
464	0/5	0/5	0/5	0/5	0/5
1000	0/5	0/5	0/5	3/5	4/5
2150	0/5	0/5	4/5	4/5	5/5
4640	0/5	0/5	4/5	5/5	-

 

Applicant's summary and conclusion

Interpretation of results:

harmful

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Under the conditions of the study, the LD50 of the test substance was determined to be 794 mg/kg/bw when dosed to male rats, in a single administration, by oral gavage.

Executive summary:

In an acute oral toxicity study performed in male albino rats, the LD50 was determined to be 794 mg/kg/bw with confidence limits of 584 to 1080 mg/kg.