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EC number: 610-472-5 | CAS number: 49707-23-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-10-27 to 2010-04-07
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study, study is performed according to OECD and EU guidelines, no deviation from study plan.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N-(2,2-dimethoxyethyl)prop-2-enamide
- EC Number:
- 610-472-5
- Cas Number:
- 49707-23-5
- Molecular formula:
- C7 H13 N1 O3
- IUPAC Name:
- N-(2,2-dimethoxyethyl)prop-2-enamide
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 1 ( 100 mg/L)
- Sampling method: no data
- Sample storage conditions before analysis: yes., storage < -10° C
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: according to OECD 202 a limit test was conducted (dissolving 41.5mg of the test item into 415mL test water by intense stirring for 20 minutes).
- Controls: In the control, test water was used without addition of the test item
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no evidende, acc. to water solubitlity the test item is fully miscible with water
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain: Clone 5
- Source: The Daphnia were bred in the laboratories of the testing facility under similar temperature and light conditions as used in the test. The test organisms were not first brood progeny.
- Age at study initiation (mean and range, SD): From 5.5 to 21.75 hours old
- Method of breeding: continous laboratory culture
- Feeding during test: no
- Food type: not applicable
- Amount: not applicable
- Frequency: not applicable
ACCLIMATION
- Acclimation period: not necessary, laboratory breed stock
- Acclimation conditions (same as test or not): see above
- Type and amount of food: Desmodesmus
- Feeding frequency: all working days
- Health during acclimation (any mortality observed): not applicable
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- no
Test conditions
- Hardness:
- Reconstituted Water, (Elendt M4):
Water Hardness: 2.5 mmol/L (= 250 mg/L) as CaCO3
Alkalinity: 0.9 mmol/L - Test temperature:
- 20 to 21 °C at test start;
20 °C at test end - pH:
- 7.9 at test start;
7.8 at test end; and thus the pH-value did not vary by more than 1.5 units - Dissolved oxygen:
- 8.7 - 8.8 mg/L at test start;
8.6 mg/L at test end - Nominal and measured concentrations:
- see block "additional information on results"
- Details on test conditions:
- TEST SYSTEM
- Test vessel: beaker, 100 ml
- Material, size, headspace, fill volume: 80 ml
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): not applicable
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): not applicable
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
- Culture medium different from test medium: no
- Intervals of water quality measurement: no data
OTHER TEST CONDITIONS
- Adjustment of pH: no data
- Photoperiod: 16/8 l/d
- Light intensity: 330 - 550 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable
- Range finding study: yes
- Test concentrations: 1-10-100 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- no
- Remarks:
- historical data
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: no
- Observations on body length and weight: no data
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: yes, in the range fining test. Therefore a solvent and lower concentrations were used in the definite test - Results with reference substance (positive control):
- - Results with reference substance valid, as determined by historical and regularily conducted study
- Mortality: control: 0
- EC50/LC50: 1.59 (24 h) and 1.374 mg/L (48 h) - Reported statistics and error estimates:
- not applicable due to no observed immobility
Any other information on results incl. tables
Nominal Concentration |
No. of Daphnia tested |
No. of immobilized Daphnia after |
% of immobilized Daphnia after |
||
[mg test item/L] |
|
24 h |
48 h |
24 h |
48 h |
Control |
20 |
0 |
0 |
0 |
0 |
100 |
20 |
0 |
0 |
0 |
0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxic effect of the test item N-(2,2-Dimethoxyethyl)acrylamide (NAAADA) to Daphnia magna was assessed in a static limit-test. The 48-hour NOEC was determined to be => 100 mg test item/L. The 48-hour EC50 value was calculated to be > 100 mg test item/L.
- Executive summary:
After 48 hours of exposure no immobilisation of the test animals was observed in the control and the only test item concentration of nominal 100 mg/L.All validity criteria were met.
The toxic effect of the test item N-(2,2-Dimethoxyethyl)acrylamide (NAAADA) to Daphnia magna was assessed in a static limit-test. The 48-hour NOEC was determined to be => 100 mg test item/L. The 48-hour EC50 value was calculated to be > 100 mg test item/L.
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