1-Ethyl-3-methylimidazolium Diethyl Phosphate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Report date:

2019

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: Chemicals - Daphnia magna acute immobilisation test

Version / remarks:

GB/T 21830-2008

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: The Guidelines for the testing of chemicals-effects on biotic systems

Version / remarks:

2nd edition, Beijing: China Environment Press, 2013

Test guideline 3

Qualifier:

according to guideline

Guideline:

other: The guidelines for the testing of chemicals

Version / remarks:

HJ/T 153-2004

Test guideline 4

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

Version of 2004

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-Ethyl-3-methylimidazolium Diethyl Phosphate

Cas Number:

848641-69-0

Molecular formula:

C6H11N2 C4H10O4P

IUPAC Name:

1-Ethyl-3-methylimidazolium Diethyl Phosphate

Test material form:

liquid

Details on test material:

Batch No 187-9-113-Y
Expiry date: 2021-1-30

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: 187-9-113-Y
- Expiration date of the lot/batch: 2021-01-30


STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Keep the container closed and in a dry, cool, well ventilated area
- Stability under test conditions:
- Solubility and stability of the test substance in the test medium: good solublility; analysed concentration was maintained above 80% of the initial measured concentration

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Final dilution of a dissolved solid, stock liquid or gel: 100 mg/L

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Study was performed as a limit test at a concentration of 100 mg/L. Sampling was done at 0 hours, 24 hours and after 48 hours. Analysis was done by UPLC-MS.

Test solutions

Vehicle:

no

Details on test solutions:

A stock solution (100 mg/L) was prepared by dissolving 0.0256 g of the test substance in 250 ml Elendt M7 medium.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna was introduced from Nanjing Institute of Environmental Science, MEP in 2018. Culture medium was Elendt M7. All organisms used for a test originated from cultures established from the same healthy stock of Daphnia. The animals were maintained under culture conditions (light, temperature and medium) similar to those used in the test. Animals used for the test were not older than 24 hours.

- Age at study initiation: <24 hours
- Feeding during test: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

20.2°C to 20.4°C

pH:

in the range of 6.87 to 7.2 without adjustment of pH during the limit test

Dissolved oxygen:

8.09 mg/L to 8.54 mg/L of the air saturation without aerating

Nominal and measured concentrations:

nominal: 100 mg/L
measured:
0h 106 mg/L
24h 104 mg/L
48h 101 mg/L

mean measured concentration: 104 mg/L

Details on test conditions:

4 replicates with 5 daphnids in 50 ml of test solution were used in the main test, open test vessels were used and no aeration was provided. The control also consists of 4 replicates with 5 daphnids each.
Photoperiod: 16:8 light-dark cycle
Number of immobilised daphnids was determined after 24 hours and 48 hours.


RANGE-FINDING STUDY
- Test concentrations: 1 mg/L, 10 mg/L and 100 mg/L
- Results used to determine the conditions for the definitive study: no immobilised daphnids were observed therefore the main study was conducted as a limit test with 100 mg/L test substance.

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

EC50 (24h) with K2Cr2O7 was 1.46 mg/L

Reported statistics and error estimates:

EC50 and 95% confidence intervals were calculated by Trimmed Spearman-Karber Method (Version 1.5, US EPA)

Any other information on results incl. tables

see attachments

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The substance is not toxic to daphnids under static conditions in a limit test with a concentration of 100 mg/L.

Executive summary:

The toxicity of the test item to Daphnia magna was investigated in a limit test with a concentration of 100 mg/l according to Chinese chemical law guidelines and OECD 202. The concentration of the test substance was stable over the test duration of 48 hours. The test fulfills the validity criteria:

• Number of immobilised daphnids in the control was <10%
• dissolved oxygen concentration was more than 3 mg/l in control and test vessels
• the pH values did not differ by more than 1.5 units at test start and end 

Under the test conditions the 48 h EC50 of the test substance was >100 mg/L.