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EC number: 203-464-4 | CAS number: 107-12-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: screening test, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Data source
Reference
- Reference Type:
- publication
- Title:
- etabolism of Acetonitrile and Propionitrile by Nocardia rhodochrous LL100-211
- Author:
- M. J. DiGERONIMO AND A. D. ANTOINE
- Year:
- 1 976
- Bibliographic source:
- DiGeronimo, M.J. & Antoine, A.D., 1976. Metabolism of acetonitrile and propionitrile by Nocardia rhodochrous LL100-21. Applied and Environmental Microbiology, 31(6), pp.900–906.
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- - Principle of test:
the biodegradation potential of propionitrile by the fungi Nocardia rhodochrous was tested.
- Short description of test conditions: cultures were grown in a basal salts broth on a gyratory shaker for 1 day at 28°C.
- Parameters analysed / observed: growth (optical density), nitrile and metabolites in the filtrate - GLP compliance:
- not specified
Test material
- Reference substance name:
- Propiononitrile
- EC Number:
- 203-464-4
- EC Name:
- Propiononitrile
- Cas Number:
- 107-12-0
- Molecular formula:
- C3H5N
- IUPAC Name:
- propanenitrile
Constituent 1
- Specific details on test material used for the study:
- obtained from Eastman Kodak, Rochester, N.Y., or from Fisher Scientific Co., Springfield, N.J. The nitriles used in this study contained less than 0.05% (wt/wt) Nes
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: basal salts broth (BSB)
- Details on inoculum:
- Cultivation and isolation of cultures:
The basal salts broth (BSB) contained (per liter): NaCl, 0.1 g; MgSO4 7H20, 0.2 g; K2HPO4, 1.03 g; KH2PO4, 0.75g, CaCl2, 25mg, ethylenediaminetetraaceticacid, 15 mg; ZnSO47H2O, 6.7mg; MnSO44H2O, 1.7mg; FeSO4-7H2O, 1.5mg;CoCl26H2O, 0.49mg; CuSO4, 0.47 mg; NaMoO4- 2H2O, 0.45 mg; pH 7.0. The MgSO4 *7H20 and CaCl2 were autoclaved separately and added aseptically to the autoclaved BSB. The isolate was obtained from barnyard soil by an enrichment culture technique using BSB (500ml/1,000-ml Erlenmeyer flask) containing 4% (wt/vol) acetonitrile as a source of both carbon and nitrogen. After a 10-day period of stationary incubation at 28°C, samples of the enrichment culture were plated on a basal salts agar (2%wt/vol) plus 4% (wt/vol) acetonitrile and incubated aerobically at 28°C. Colonies were selected and reinoculated into the BSB-acetonitrile medium for confirmation of its ability to grow on acetonitrile. - Duration of test (contact time):
- 1 d
Initial test substance concentration
- Initial conc.:
- 10 g/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- other: nitrile concentration
- Details on study design:
- Substrate screening:
Cells were inoculated into 20-ml culture tubes containing 5 ml of BSB with the test compound (10 mg/ml). The growth control tubes contained 10 mg of sodium acetate per ml and 0.05 mg of ammonium sulfate per ml in place of a nitrile or amide. The inoculum was prepared by grwoing the isolate in BSB (100 ml/250 ml Erlenmeyer flask) containing 10 mg of acetonitrile per ml on a gyratory shaker, 200 rpm for 18h. The cells were harvested by centrifugation at 12 000 x g for 10 min at 5°C and resuspended in sterile 0.1 M potassium phosphate buffer (pH 7.2). A 5% (vol/vol) inoculum of this cell suspension and an incubation temperature of 28°C were used throughout the study.
Whole-cell studies:
For metabolic studies,cultures were grown on a gyratory shaker (200 rpm) in Fernbach flasks containing 1 liter of BSB and 10 mg of the nitrile per ml tested. Samples (5 ml) were removed from the culture broth periodically during the growth cycle. The sample was vacuum filtered through a 0.45-µm membrane filter (Millipore Corp., Bedford, Mass.) to remove the cells, and the filtrate was analyzed for nitrile concentration and for the presence of any detectabel metabolites. Growth was measured by optical density readings using a Coleman spectrophotometer (model 295) at 600 nm.
Cell-free studies:
Cell-free extracts were prepared by growing the isolate in 1-liter volumes of the BSB-acetonitrile (10 mg/ml) medium and harvesting the
cells in mid-log phase (18 h) by centrifugation with a steam-driven Sharples at 30,000 rpm. The packed cells (4 g of wet wt/liter of medium) were suspended in 2 ml ofcold 0.1 M potassium phosphate buffer (pH 7.2) per g of wet weight of biomass. The cells were disrupted in a Raytheon 10-Kc sonic oscillator for 10 min with 10 g of glass beads (no. 812 Cataphote, Cataphote Corp., Toledo, Ohio) for every 25 ml of cell suspension. Following sonification, the mixture was centrifuged at 4,300 x g for 3 min to remove the glass beads and unbroken cells. The supernatant fluid was then centrifuged at 35 000 x g for 15 min, which resulted in a clear supernatant fluid (typical protein concentration was 28 mg/ml). The supernatant fluid (crude extract) was stored at -25 °C. Assay of the crude extract for enzymatic activity was based on the detection of the substrates and products by GLC and colorimetric procedures. In the assay, 3.0 ml of 0.1 M potassium phosphate buffer (pH 7.2) containing 10 mg of acetonitrile per ml (0.244 mmol/ml) was equilibrated to 37°C, and 0.1 to 0.5 ml of the cell-free extract was added to start the reaction. The reaction was stopped by adding 1 ml of cold 10% trichloroacetic acid at various time intervals. The reaction mixture was then centrifuged at 3,000 rpm for 5 min (Sorvall, model no. GLC-1), and the supernatant fluid was analyzed.
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 100
- Sampling time:
- 96 h
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 11
- Sampling time:
- 24 h
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- Complete biodegradation of propionitrile by Nocardia rhodochrous after 96 hours in synthetic medium was observed.
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