Methyl nicotinate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

This study was designed to assess the toxic effects of the test compound on the ZEBRA FISH(Danio rerio) .Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes each. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. The experimental phase of the main study started at 27-02-2017 and lasted for a period of 96 hrs.

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material (as cited in study report):Methyl nicotinate
- Molecular formula (if other than submission substance):C7H7NO2
- Molecular weight (if other than submission substance):137.137 g/mol
- Substance type:Organic
- Physical state:white solid powder

Analytical monitoring:

not specified

Vehicle:

no

Details on test solutions:

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with 1 hr continuous stirring for achieving test concentrations of 100mg/L ,respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:ZEBRA FISH
- Source:UNIQUE AQUARIUM, NAGPUR
- Length at study initiation (length definition, mean, range and SD):average 1.63 cm
- Weight at study initiation (mean and range, SD):0.1899g (average)

ACCLIMATION
- Acclimation period:7 days
- Type and amount of food during acclimation:Standard brand feed
- Feeding frequency during acclimation:Daily once
- Health during acclimation (any mortality observed):During last 48 hours = 0

FEEDING DURING TEST
- Food type:Standard brand feed
- Frequency:Withdrawal of food 1 day before and during exposure

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

148.5 mg of CaCO3

Test temperature:

23.5 °C

pH:

7.15

Dissolved oxygen:

7.9 mg/l

Nominal and measured concentrations:

control and 100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel:The test vessels used were all plastic aquaria having the water capacity of 5liters.
- Material, size, headspace, fill volume: 2 liters
- No. of organisms per vessel:8 fishes

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Mortality and symptoms:

Nominal Concentration (mg/l)	Number of fish		Mortality (cumulated) after
			3 h		6 h		24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	-	-	-	-	-	-	-	-	-	-	-	-
100	4	4	-	-	-	-	-	-	-	-	-	-	-	-

 

Visible Symptoms:

Nominal Concentration (mg/l)

Number of fish

symptom (cumulated) after

3 h

6 h

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

100

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

Validity criteria fulfilled:

yes

Conclusions:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for (test item name) Methyl nicotinate. Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No.93-60-7 on ZEBRA FISH(Danio rerio)was found to be >100 mg/L.

Executive summary:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for (test item name) Methyl nicotinate. The nominal concentration selected for the experiment were 100 mg/Land test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >100 mg/L as no mortality was observed during the experiment.

Description of key information

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for (test item name) Methyl nicotinate The nominal concentration selected for the experiment were 100 mg/Land test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >100 mg/L since no mortality was observed during the experiment.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Four studies including those from experimental study and validated prediction for short term fish toxicity endpoint of target chemical Methyl nicotinate (Cas no. 93-60-7) with relevant read across which is structurally similar to target were summarized as followed: 

 

First experimental key study by UERL laboratory; 2017 suggest the Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Methyl nicotinate, The nominal concentration selected for the experiment were 100mg/Land test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >100 mg/L since no mortality was observed at 100 mg/l in 96 hrs exposure period.

 

Above aquatic classification is supported by prediction for target, from EPI Suite ECOSAR version 1.10 wherein the 96 hours LC50 was estimated to be 1876.926 mg/l on Fish on the basis of mortality effects using neutral organics class for prediction. Based on this value, it can be concluded that the test chemical Methyl nicotinate can be considered as non toxic to fish at mentioned predicted lethal concentration thus it can be considered not classified as per the CLP classification criteria.

Another predicted result from Danish QSAR database reported the 96 hrs LC50 value by three different models i.e, Battery, Leadscope and SciQSAR, used within the database, to be 168.31 mg/l for test chemical Methyl nicotinate.

All the above studies for target chemical are further supported by read across Nicotinic acid (Cas no. 59-67-6). Acute fish toxicity according to OECD Guideline 203 was conducted for chemical Nicotinic acid (Cas no. 59-67-6). According to toxicity study the median lethal concentration (LC50) for 96 hrs.exposure period was observed to be 520 mg/l on fish species Brown trout (Salmo trutta) (Screening Information Data Set (SIDS) of OECD High Production Volume Chemicals Programme, (1993))

Thus based on all available studies for target and read across chemical gives the lethal concentration is in the range of 100-1876.926 mg/l concluded that the test Methyl nicotinate (Cas no. 93-60-7) considered as non-toxic to fish at environmentally relevent concentrations and thus cannot be classified as hazardous as per the CLP criteria.