7,16-dichloro-6,15-dihydroanthrazine-5,9,14,18-tetrone

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

12 March 1974

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

No information given on results with negative control.

Qualifier:

according to guideline

Guideline:

other: The test was conducted according to SOP B-10 (1977-09-01).

Principles of method if other than guideline:

- Principle of test: Determination of an acute bacteriostatic toxicity to activated sludge und aerobic conditions.
- Short description of test conditions: Bacteria suspension from activated sludge filtrate is incubated for 12 - 24 h at 25°C in suspension tubes with nutrient broth (OECD).
- Parameters analysed / observed: Growth inhibition is determined visually (turbidity). The toxicity to activated sludge is determined as the lowest effect concentration where no growth is observed compared to the negative control.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution is diluted to 1, 5, 10, 20, 50, 100, 200 and 300 ppm dilutions. 0.1 mL of each dilution is mixed with 4.5 mL nutrient broth in a test tube.
- Controls: negative control, solvent control if solvent used
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Methyl cellulose (<2%) or dimethyl formamide (<1%) if not water soluble

Test organisms (species):

activated sludge

Details on inoculum:

- Laboratory culture: yes
- Method of cultivation: activated sludge bred in a aerated Husmann apparatus, weekly preparation
- Preparation of inoculum for exposure: 100 mL activated sludge from day 3 to 5 are taken from the effluent and filtrated with Whatman GF/A.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

25 +/- 2°C

Nominal and measured concentrations:

Nominal: 1, 5, 10, 20, 50, 100, 200, 300 ppm

Details on test conditions:

TEST SYSTEM
- Test vessel: tube
- Material, size, headspace, fill volume: glass
- Nutrients provided for bacteria: nutrient broth (OECD)
- Test tubes provided with nutrient broth and the respective test substance concentration are inoculated with 1 drop of filtrated bacteria suspension from activated sludge.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

> 300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Validity criteria fulfilled:

not applicable

Conclusions:

The 48-h ErC0 for bacteria from activated sludge was determined to be >300 mg/L.

Reference 2

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

28 November 1977

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

No information given on results with negative control.

Qualifier:

no guideline available

Version / remarks:

The test was conducted according to SOP B-10 (1977-09-01).

Principles of method if other than guideline:

- Principle of test: Determination of an acute bacteriostatic toxicity to activated sludge und aerobic conditions.
- Short description of test conditions: Bacteria suspension from activated sludge filtrate is incubated for 12 - 24 h at 25°C in suspension tubes with nutrient broth (OECD).
- Parameters analysed / observed: Growth inhibition is determined visually (turbidity). The toxicity to activated sludge is determined as the lowest effect concentration where no growth is observed compared to the negative control.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution is diluted to 1, 5, 10, 20, 50, 100, 200 and 300 ppm dilutions. 0.1 mL of each dilution is mixed with 4.5 mL nutrient broth in a test tube.
- Controls: negative control, solvent control if solvent used
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Methyl cellulose (<2%) or dimethyl formamide (<1%) if not water soluble

Test organisms (species):

activated sludge

Details on inoculum:

- Laboratory culture: yes
- Method of cultivation: activated sludge bred in a aerated Husmann apparatus, weekly preparation
- Preparation of inoculum for exposure: 100 mL activated sludge from day 3 to 5 are taken from the effluent and filtrated with Whatman GF/A.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

25 +/- 2°C

Nominal and measured concentrations:

Nominal: 1, 5, 10, 20, 50, 100, 200, 300 ppm

Details on test conditions:

TEST SYSTEM
- Test vessel: tube
- Material, size, headspace, fill volume: glass
- Nutrients provided for bacteria: nutrient broth (OECD)
- Test tubes provided with nutrient broth and the respective test substance concentration are inoculated with 1 drop of filtrated bacteria suspension from activated sludge.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Validity criteria fulfilled:

not applicable

Conclusions:

The 48-h ErC0 for bacteria from activated sludge was determined to be >300 mg/L.

Reference 3

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

24 May 1978

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

No information given on results with negative control.

Qualifier:

no guideline available

Version / remarks:

The test was conducted according to SOP B-10 (1977-09-01).

Principles of method if other than guideline:

- Principle of test: Determination of an acute bacteriostatic toxicity to activated sludge und aerobic conditions.
- Short description of test conditions: Bacteria suspension from activated sludge filtrate is incubated for 12 - 24 h at 25°C in suspension tubes with nutrient broth (OECD).
- Parameters analysed / observed: Growth inhibition is determined visually (turbidity). The toxicity to activated sludge is determined as the lowest effect concentration where no growth is observed compared to the negative control.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution is diluted to 1, 5, 10, 20, 50, 100, 200 and 300 ppm dilutions. 0.1 mL of each dilution is mixed with 4.5 mL nutrient broth in a test tube.
- Controls: negative control, solvent control if solvent used
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Methyl cellulose (<2%) or dimethyl formamide (<1%) if not water soluble

Test organisms (species):

activated sludge

Details on inoculum:

- Laboratory culture: yes
- Method of cultivation: activated sludge bred in a aerated Husmann apparatus, weekly preparation
- Preparation of inoculum for exposure: 100 mL activated sludge from day 3 to 5 are taken from the effluent and filtrated with Whatman GF/A.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Nominal and measured concentrations:

Nominal: 1, 5, 10, 20, 50, 100, 200, 300 ppm

Details on test conditions:

TEST SYSTEM
- Test vessel: tube
- Material, size, headspace, fill volume: glass
- Nutrients provided for bacteria: nutrient broth (OECD)
- Test tubes provided with nutrient broth and the respective test substance concentration are inoculated with 1 drop of filtrated bacteria suspension from activated sludge.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

> 300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Validity criteria fulfilled:

not specified

Conclusions:

The 48-h ErC0 for bacteria from activated sludge was determined to be >300 mg/L.

Description of key information

Three 48 -h ErC0s were determined to be >300 mg/L for bacteria suspensions from activated sludge. No toxic effect to bacteria was observed up to the water solubility limit (< 1.0 mg/L) based on a weight-of-evidence approach.