7,16-dichloro-6,15-dihydroanthrazine-5,9,14,18-tetrone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

21 - 23 February 1974

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

No information on negative control available.

Qualifier:

no guideline available

Version / remarks:

The test was conducted according to SOP A-11 (1977-09-01).

Principles of method if other than guideline:

- Principle of test: Fish are exposed to different solutions of the test substance.
- Short description of test conditions: 8-10 fish are used per concentration. Synthetic water is used, test concentrations are fixed.
- Parameters analysed / observed: Mortality (LC0, LC100), LC50 derivation is performed graphically and/or mathematically.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: dilution of a stock solution with synthetic water according to ISO norms
- Differential loading: 100, 200, 1000 mg/L
- Controls: positive control, solvent control if solvent used
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Methyl cellulose (2%) or ethanol (0.3%) or acetone (0.6%) if not water soluble

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Source: P. Hohler, 4314 Zeiningen, Germany
- Age at study initiation: born in summer 1973
- Length at study initiation: 4-8 cm
- Weight at study initiation: 3-6 g

ACCLIMATION
- Acclimation period: min. 12 d
- Acclimation conditions (same as test or not): 10-13°C in flow-through aquaria with dechlorinated tap water, O2 min. 6.4 mg/L, day/night 12/12 h
- Type and amount of food during acclimation: Tetramin flakes
- Feeding frequency during acclimation: daily

FEEDING DURING TEST
- Frequency: no feeding during test

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

12°C

pH:

8.0 - 8.2

Dissolved oxygen:

>4.8 mg/L

Nominal and measured concentrations:

Nominal: 100, 200, 1000 mg/L

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 8
- No. of vessels per concentration (replicates): 1

TEST SYSTEM
- Test vessel: aquaria
- Material, size, headspace, fill volume: glass, 36 x 23 x 26 cm (17 L)
- Aeration: yes
- No. of organisms per vessel: 8
- No. of vessels per concentration (replicates): 1
- No. of vessels per vehicle control (replicates): 1 (if applicable)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Culture medium different from test medium: yes

OTHER TEST CONDITIONS
- Adjustment of pH: yes (if not in range of 6 - 8.5)
- Photoperiod: 12/12 h

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality at 24, 48, 72 and 96 h. 72 and 96 h only observed if effects observed at earlier test stages.

Reference substance (positive control):

yes

Remarks:

Fish are randomly tested for sensitivity 6 d after arrival from the source (test substance: HgCl2).

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Not acutely toxic to fish with water from effluent (wastewater treatment plant) diluted by factor 5.

Validity criteria fulfilled:

not applicable

Conclusions:

The 48-h LC50 to fish (Oncorhynchus mykiss) was determined to be >1000 mg/L.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

16 - 18 December 1977

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

No information on negative control available.

Qualifier:

no guideline available

Version / remarks:

The test was conducted according to SOP A-11 (1977-09-01).

Principles of method if other than guideline:

- Principle of test: Fish are exposed to different solutions of the test substance.
- Short description of test conditions: 8-10 fish are used per concentration. Synthetic water is used, test concentrations are fixed.
- Parameters analysed / observed: Mortality (LC0, LC100), LC50 derivation is performed graphically and/or mathematically.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: dilution of a stock solution with synthetic water according to ISO norms
- Differential loading: 100, 200, 1000 mg/L
- Controls: positive control, solvent control if solvent used
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Methyl cellulose (2%) or ethanol (0.3%) or acetone (0.6%) if not water soluble

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Source: P. Hohler, 4314 Zeiningen, Germany
- Age at study initiation: born in summer 1973
- Length at study initiation: 4-8 cm
- Weight at study initiation: 3-6 g

ACCLIMATION
- Acclimation period: min. 12 d
- Acclimation conditions (same as test or not): 10-13°C in flow-through aquaria with dechlorinated tap water, O2 min. 6.4 mg/L, day/night 12/12 h
- Type and amount of food during acclimation: Tetramin flakes
- Feeding frequency during acclimation: daily

FEEDING DURING TEST
- Frequency: no feeding during test

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

14°C

pH:

8.0 - 8.2

Dissolved oxygen:

>4.8 mg/L

Nominal and measured concentrations:

Nominal: 100, 200, 1000 mg/L

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 8
- No. of vessels per concentration (replicates): 1

TEST SYSTEM
- Test vessel: aquaria
- Material, size, headspace, fill volume: glass, 36 x 23 x 26 cm (17 L)
- Aeration: yes
- No. of organisms per vessel: 8
- No. of vessels per concentration (replicates): 1
- No. of vessels per vehicle control (replicates): 1 (if applicable)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Culture medium different from test medium: yes

OTHER TEST CONDITIONS
- Adjustment of pH: yes (if not in range of 6 - 8.5)
- Photoperiod: 12/12 h

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality at 24, 48, 72 and 96 h. 72 and 96 h only observed if effects observed at earlier test stages.

Reference substance (positive control):

yes

Remarks:

Fish are randomly tested for sensitivity 6 d after arrival from the source (test substance: HgCl2).

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

not applicable

Conclusions:

The 48-h LC50 to fish (Oncorhynchus mykiss) was determined to be >1000 mg/L.

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

21 - 23 April 1978

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

No information on negative control available.

Qualifier:

no guideline available

Version / remarks:

The test was conducted according to SOP A-11 (1977-09-01).

Principles of method if other than guideline:

- Principle of test: Fish are exposed to different solutions of the test substance.
- Short description of test conditions: 8-10 fish are used per concentration. Synthetic water is used, test concentrations are fixed.
- Parameters analysed / observed: Mortality (LC0, LC100), LC50 derivation is performed graphically and/or mathematically.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: dilution of a stock solution with synthetic water according to ISO norms
- Differential loading: 100, 200, 1000 mg/L
- Controls: positive control, solvent control if solvent used
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Methyl cellulose (2%) or ethanol (0.3%) or acetone (0.6%) if not water soluble

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Source: P. Hohler, 4314 Zeiningen, Germany
- Age at study initiation: born in summer 1973
- Length at study initiation: 4-8 cm
- Weight at study initiation: 3-6 g

ACCLIMATION
- Acclimation period: min. 12 d
- Acclimation conditions (same as test or not): 10-13°C in flow-through aquaria with dechlorinated tap water, O2 min. 6.4 mg/L, day/night 12/12 h
- Type and amount of food during acclimation: Tetramin flakes
- Feeding frequency during acclimation: daily

FEEDING DURING TEST
- Frequency: no feeding during test

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

14°C

pH:

7.9 - 8.1

Dissolved oxygen:

>4.8 mg/L

Nominal and measured concentrations:

Nominal: 100, 200, 1000 mg/L

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 8
- No. of vessels per concentration (replicates): 1

TEST SYSTEM
- Test vessel: aquaria
- Material, size, headspace, fill volume: glass, 36 x 23 x 26 cm (17 L)
- Aeration: yes
- No. of organisms per vessel: 8
- No. of vessels per concentration (replicates): 1
- No. of vessels per vehicle control (replicates): 1 (if applicable)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Culture medium different from test medium: yes

OTHER TEST CONDITIONS
- Adjustment of pH: yes (if not in range of 6 - 8.5)
- Photoperiod: 12/12 h

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality at 24, 48, 72 and 96 h. 72 and 96 h only observed if effects observed at earlier test stages.

Reference substance (positive control):

yes

Remarks:

Fish are randomly tested for sensitivity 6 d after arrival from the source (test substance: HgCl2).

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Not acutely toxic to fish with water from effluent (wastewater treatment plant) diluted by factor 5.

Validity criteria fulfilled:

not applicable

Conclusions:

The 48-h LC50 to fish (Oncorhynchus mykiss) was determined to be >1000 mg/L.

Description of key information

Three 48-h LC50s were determined to be >1000 mg/L for Oncorhynchus mykiss. No acutely toxic effect to fish was observed up to the water solubility limit (< 1.0 mg/L) based on a weight-of-evidence approach.