Sodium 4-vinylbenzenesulphonate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 July to 7 August 1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Benzenesulfonic acid, 4-ethenyl-, sodium salt (1:1)

IUPAC Name:

Benzenesulfonic acid, 4-ethenyl-, sodium salt (1:1)

Constituent 2

Reference substance name:

Sodium 4-vinylbenzenesulphonate

EC Number:

220-266-3

EC Name:

Sodium 4-vinylbenzenesulphonate

Cas Number:

2695-37-6

Molecular formula:

C8H8O3S.Na

IUPAC Name:

.

Constituent 3

Reference substance name:

Sodium p-styrenesulfonate

IUPAC Name:

Sodium p-styrenesulfonate

Constituent 4

Reference substance name:

Spinomar NaSS

IUPAC Name:

Spinomar NaSS

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report):Spinomar NaSS
- Substance type: Organic
- Physical state:solid
- Lot/batch No.:
- Stability under test conditions: stable
- Storage condition of test material: room temperature
- Other:

Method

Target gene:

Histidine-requiring S. tryphimurium mutants TA 1535, TA 1537, TA 98 and TA 100 used in the test were provided by Dr. B.N.Ames, University of California Berkeley, California, U.S.A.

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Metabolic activation system:

S-9 used in this test was part of a batch prepared. This batch was prepared from the livers of rodents treated with Arclor 1254 in soya bean oil.

Test concentrations with justification for top dose:

62, 185, 556, 1667, 5000 microgram/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: water
- Justification for choice of solvent/vehicle: test substance dissolves freely in the water.

Details on test system and experimental conditions:

METHOD OF APPLICATION: in medium

DURATION
- Preincubation period:
- Exposure duration:3 days
- Expression time (cells in growth medium):
- Selection time (if incubation with a selection agent):
- Fixation time (start of exposure up to fixation or harvest of cells):

Evaluation criteria:

A concentration-related increase over the range tested and/or a reproducible increase at one or more concentrations in the number of revertant colonies per plaste in at least one strain with or without metabolic activation systems.

Results and discussion

Test results

Key result

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Under the condictions of the Ames test conducted on S. typhimurium TA 1535, TA 1537, TA 98 and TA 100 no mutagenic activity was observed.

Executive summary:

Mutagenicity potential of NaSS was assessed with Salmonella typhimurium TA100, TA1535, TA98 and TA1537 according to the OECD 471 test guideline compliant with GLP.

The substance did not induce an increase in the number of revertant colonies more than twice in comparison with that of the negative control nor was a dose-related response observed in any strains of base-pair substitution type or frame-shift type, with or without metabolicactivation. 

Under the conditions of the test the substance is not mutagenic.